1 / 13

Dr.Chen Gao cg_npwr@yahoo

DNA 的提取与含量测定. Dr.Chen Gao cg_npwr@yahoo.com.cn. DNA ， DNP. 变性蛋白，肝糜. 氯仿. 100 ℃. 蓝色物. DNA +. 冰醋酸，少量硫酸. ( 二 ) 二苯胺法测定 DNA 含量. 二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和分别针对 DNA 和 RNA 的颜色反应方法 本实验采用针对 DNA 的二苯胺法测 DNA 的含量。在酸性溶液中， DNA 与二苯胺共热生成蓝色化合物，该物质在 595nm 有最大吸收，在 40 ～ 400mg/mL 范围内其峰值与 DNA 含量成正比.

cade
Download Presentation

Dr.Chen Gao cg_npwr@yahoo

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. DNA的提取与含量测定 Dr.Chen Gao cg_npwr@yahoo.com.cn CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  2. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  3. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  4. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  5. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  6. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  7. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  8. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  9. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  10. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  11. DNA，DNP 变性蛋白，肝糜 氯仿 CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  12. CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

  13. 100℃ 蓝色物 DNA + 冰醋酸，少量硫酸 (二)二苯胺法测定DNA含量 二、原理 核酸含量的测定方法包括紫外吸收法、定磷法和分别针对DNA和RNA的颜色反应方法 本实验采用针对DNA的二苯胺法测DNA的含量。在酸性溶液中，DNA与二苯胺共热生成蓝色化合物，该物质在595nm有最大吸收，在40～400mg/mL范围内其峰值与DNA含量成正比 CECS 694-02 Introduction to Bioinformatics University of Louisville Spring 2004 Dr. Eric Rouchka

More Related