1 / 3

100

ActD 0h. ActD 6h. 120.0. 100.0. 80.0. (% of ActD 0h). Reporter activity. 60.0. 40.0. 20.0. 0.0. AANAT. -. 3‘UTR. 1263. 449. 1889. 120. 100. 80. % of activity remaining. 60. 40. NAT. 3'U1889. 20. 3'U1263. 0. 0. 0.5. 1. 1.5. 2. [h]. Woo et al_.

caesar
Download Presentation

100

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. ActD 0h ActD 6h 120.0 100.0 80.0 (% of ActD 0h) Reporter activity 60.0 40.0 20.0 0.0 AANAT - 3‘UTR 1263 449 1889 120 100 80 % of activity remaining 60 40 NAT 3'U1889 20 3'U1263 0 0 0.5 1 1.5 2 [h] Woo et al_ mper2 3′UTR end sequence is responsible for its own degradation Supplementary Fig. S1 A B

  2. + 3′U1889 3′U1877 + IP + 3′U1263 + 3′U1660 3′U449 + + + + 3′U1440 + PTB + Ab 3′U1263 #1 + + + + competitors SCN-N SCN-N + + + + + + 90.5 90.5 90.5 61.5 61.5 61.5 46.2 46.2 46.2 37.8 37.8 37.8 + + 3′U449 #1 competitor + + + 3′U1889 + + + + + 3′U449 + S100 + + ++ Cold-3′U449 purified-PTB4 (ng) 25 400 + + + NIH3T3-c 90.5 90.5 61.5 61.5 46.2 46.2 37.8 26 Woo et al_ PTB binds to pyrimidine tract in mper2 3′end sequence in SCN Supplementary Fig. S2 A B PIS C E D

  3. Cyto. Nuc. 6 18 6 18 12 24 12 24 PTB hnRNP D (short Exp.) hnRNP D (long Exp.) 9 18 21 0 6 12 14 16 Cyto. Nuc. 18 0 6 12 14 16 Lamin B 14-3-3ξ Woo et al_ Cytoplasmic PTB differentially translocalizes at respective phase Supplementary Fig. S3 A B

More Related