featured-products-for-cellular-neuroscience-research

1. Featured Products for Cellular Neuroscience Research https://neuros.creative-biolabs.com

2. TO PROMOTE NEUROSCIENCE RESEARCH TO BUILD A HEALTHIER TOMORROW

3. 01 Overview Our Mission Cellular Neuroscience Tools for Cellular Neuroscience Research 02 GPCR Stable Cell Line Introduction of GPCRs Classification of GPCRs GPCR Stable Cell Line Products Applications & Features 03 Sensory Neuron Culture Introduction of Sensory Neuron Types & Functions of Sensory Neuron NeuSenso™ Media Products 04 Calcium & Glutamate Signaling Genetically Encoded Calcium Indicators Genetically Encoded Glutamate Indicators Ready to Use AAV Biosensors 03

4. Everything we do is rooted in neuroscience. Creative Biolabs is a biotechnology company that focuses on discovering, developing, manufacturing and delivering innovative, high-quality scientific tools for world-wide neuroscientists. Particularly, our vision is to enable our customers to achieve outstanding success and breakthroughs in the principles of serious neurology disorders such as Amyotrophic Lateral Sclerosis (ALS, also known as Lou Gehrig’s disease), Multiple Sclerosis (MS) and Parkinson’s Disease (PD). We thereby make improvements in life possible. https biolabs.com/support/brochure/ne ural com.pdf 01 Overview 04

5. Cellular neuroscience is the study of neurons at the cellular level, including cell morphology and physiological properties. It examines various types of neurons, the function of different neurons, and the interaction between neurons. There are over 8 billion neurons in the human brain, and they are diverse with respect to morphology and functions. The function of neurons is to receive, propagate and transmit electrochemical impulses. The study of neurons at the cellular level provides insight into how neurons carry out their function, the mechanism of signal transductions and the primary cause of neurological diseases. GPCR Stable Cell Lines for Drug Discovery GPCRs are involved in a wide variety of physiological processes and diseases. The pharmaceutical industry uses GPCR stable cell lines in target discovery, target validation, assay development, and compound screening. They are also used in academia to evaluate the mechanisms of biological processes. Genetically Encoded Calcium Indicators for Calcium Imaging In neurons, electrical activity is always accompanied by an influx of Ca2+ ions. Thus, calcium imaging can be used to visualize and decode the electrical activity in hundreds of neurons in cell culture or living animals, which has made it possible to dissect the function of neuronal circuits, as well as to investigate the roles that calcium ions have in functioning neurons. Neural Cell Culture System Neuronal cultures are indispensable in the fields of neuroscience and pharmacology. The ability to produce in vitro cultures of neuronal cells has been fundamental for advancing our understanding of the functions of the nervous system. We specialize in providing the highest quality media supplements, adhesion substrates, verification antibodies, and neuromodulators for neuronal cell culture research. 01 Overview 05

6. G transmembrane receptors (7-TM receptors), are the largest family of membrane receptors with nearly 1,000 presented in the human genome. They are found only in eukaryotes (e.g., yeast, plants and animals), and can be activated by neurotransmitters, hormones, pheromones, odors, etc. Being involved in diseases such as cancers, immunological disorders, metabolic disorders, cardiovascular diseases, and mental illnesses, GPCRs are an important drug targets and estimated to be targets of 50% of drugs currently on the market. Protein-coupled Receptors (GPCRs), also konwn as 7- There are two signaling transduction pathways involving GPCRs: the cAMP signaling pathway and the phosphatidylinositol signaling pathway. The mechanism of the signaling pathways includes the activation of GPCR by an external signal in the form of a ligand, which creates a conformational change in the receptor, thus, causing activation of a G protein (guanine nucleotide-binding proteins). Class Receptor Family Name Type of Ligand Class A (or 1) Rhodopsin-like receptors Hormone, Neuropeptide, Neurotransmitter, and Light Class B (or 2) Secretin family receptors Hormone (e.g., secretin and glucagon) Class C (or 3) Metabotropic glutamate/pheromone receptors Glutamate Class D (or 4) Fungal mating pheromone receptors Mating factors Class E (or 5) Cyclic AMP receptors cAMP Class F (or 6) Frizzled/Smoothened receptors Wnt proteins/Hedgehog (Hh) One of the great features of GPCRs is that they are druggable. Despite that, only a small percentage of the known and verified GPCRs have been targeted for therapeutics. Hence, there remains enormous scope for researchers to delineate the numerous roles of GPCRs in basic physiology and pathophysiology, and to thereby understand and develop new treatments for disease. 02 GPCR Stable Cell Line 06

7. Customer-focus, Research-driven GPCR Stable Cell Line Engineering Service Stable cell lines play an important role in the treatment of many diseases, especially neurological disorders. As a trusted product and service provider, Creative Biolabs is dedicated to offering a wide spectrum of GPCR stable cell lines for various applications including analysis of different protein expressions at the cell surface. With extensive experience and advanced technologies, we have gained a reputation among our customers accomplishing numerous challenging projects in the research field of neuroscience. worldwide for Besides, Creative Biolabs has developed a well-established codon optimization and vector design platform to improve the performance of our GPCR stable cell lines. All the products from us meet the requirement of authority and the data provided by us is highly accurate and reproducible. With us, you are guaranteed with cost-effective products and services, and satisfactory progress of your project. • Identifying ligands of GPCR and studying the mechanism of the ligand-receptor interaction. • Screening for agonists or antagonists for GPCRs. • High through-put compound screening for related neurological diseases. • Broad Receptor Coverage: Over 60 GPCR families with over 130 members. • Genetic Stability: No significant changes for at least 10 passages. • No Contamination: No contamination from neither other cell lines, or mycoplasma. 02 GPCR Stable Cell Line 07

8. Family Receptor Product Name Cat.No. 5-HT1A/HTR1A Human Recombinant 5-HT1A Serotonin Receptor Stable Cell Line NCL20120001CR 5-HT2A/HTR2A Human Recombinant 5-HT2A Serotonin Receptor Stable Cell Line NCL20120002CR 5-Hydroxytryptamine receptor 5-HT2B/HTR2B Human Recombinant 5-HT2B Serotonin Receptor Stable Cell Line NCL20120003CR 5-HT2C/HTR2C Human Recombinant 5-HT2C Serotonin Receptor Stable Cell Line NCL20120004CR HTR4 Human Recombinant 5-HT4 Serotonin Receptor Stable Cell Line NCL20120006CR Human Recombinant Muscarinic Acetylcholine Receptor M1 Stable Cell Line M1/CHRM1 NCL20120007CR Human Recombinant Muscarinic Acetylcholine Receptor M2 Stable Cell Line M2/CHRM2 NCL20120008CR Acetylcholine receptor (muscarinic) Human Recombinant Muscarinic Acetylcholine Receptor M3 Stable Cell Line M3/CHRM3 NCL20120009CR Human Recombinant Muscarinic Acetylcholine Receptor M4 Stable Cell Line M4/CHRM4 NCL20120010CR Human Recombinant Muscarinic Acetylcholine Receptor M5 Stable Cell Line M5/CHRM5 NCL20120011CR ADORA1 Human Recombinant Adenosine A1 Receptor Stable Cell Line NCL20120012CR ADORA2a Human Recombinant Adenosine A2A Receptor Stable Cell Line NCL20120014CR Adenosine receptor ADORA2a Mouse Recombinant Adenosine A2A Receptor Stable Cell Line NCL20120015CR ADORA2B Human Recombinant Adenosine A2B Receptor Stable Cell Line NCL20120016CR ADORA3 Human Recombinant Adenosine A3 Receptor Stable Cell Line NCL20120017CR ADRA1B Human Recombinant ADRA1B Adrenoceptors Stable Cell Line NCL20120018CR ADRA1D Human Recombinant ADRA1D Adrenoceptors Stable Cell Line NCL20120019CR ADRA2A Human Recombinant ADRA2A Adrenoceptors Stable Cell Line NCL20120020CR Adrenoceptors ADRA2B Human Recombinant ADRA2B Adrenoceptors Stable Cell Line NCL20120021CR ADRA2C Human Recombinant ADRA2C Adrenoceptors Stable Cell Line NCL20120022CR ADRB1 Human Recombinant ADRB1 Adrenoceptors Stable Cell Line NCL20120023CR ADRA1A Human Recombinant ADRA1A Adrenoceptors Stable Cell Line NCL20120024CR AT1 Human Recombinant Angiotensin Receptor 1 Stable Cell Line NCL20120025CR Angiotensin receptor AT1 Human Recombinant Angiotensin Receptor 1 Stable Cell Line NCL20120026CR Apelin receptor AGTRL1 Human Recombinant Apelin Receptor Stable Cell Line NCL20120027CR Bile acid receptor GPBAR1 Human Recombinant Bile Acid Receptor 1 Stable Cell Line NCL20120028CR (Continuing…) 02 GPCR Stable Cell Line 08

9. Family Receptor Product Name Cat.No. BB1 Human Recombinant BB1 Bombesin Receptor Stable Cell Line NCL20120029CR Bombesin receptor BB2/GRPR Human Recombinant BB2 Bombesin Receptor Stable Cell Line NCL20120030CR BB3/BRS3 Human Recombinant BB3 Bombesin Receptor Stable Cell Line NCL20120031CR Bradykinin receptor B2 Human Recombinant B2 Bradykinin Receptor Stable Cell Line NCL20120032CR AMY1 Human CHO-K1/Gα15/AMY1 Stable Cell Line NCL20120033CR Calcitonin AMY2 Human CHO-K1/Gα15/AMY2 Stable Cell Line NCL20120034CR AMY3 Human CHO-K1/Gα15/AMY3 Stable Cell Line NCL20120035CR Calcitonin receptor CALCR/CT Human Recombinant Calcitonin Receptor Stable Cell Line NCL20120036CR CNR1/CB1 Human Recombinant Cannabinoid Receptor 1 Stable Cell Line NCL20120037CR Cannabinoid receptor CB2 Human Recombinant Cannabinoid Receptor 2 Stable Cell Line NCL20120038CR Chemerin receptor CMKLR1 Human Recombinant Chemokine Receptor-like 1 Stable Cell Line NCL20120039CR CCR1 Human chemokine (C-C motif) receptor 1 (CCR1) Stable Cell Line NCL20120040CR CCR5 Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line NCL20120041CR CXCR1/IL8RA Human chemokine (C-C motif) receptor 5 (CCR5) Stable Cell Line NCL20120042CR Chemokine receptor CXCR3 Human chemokine (C-X-C motif) receptor 3 (CXCR3A) Stable Cell Line NCL20120043CR CXCR4 Human Recombinant CXCR4 Receptor Stable Cell Line NCL20120044CR CXCR5/BLR1 Human chemokine (C-X-C motif) receptor 5 (CXCR5) Stable Cell Line NCL20120045CR CCKA/CCKAR Human Recombinant CCKA Cholecystokinin Receptor Stable Cell Line NCL20120046CR Cholecystokinin receptor CCKB/CCKBR Human Recombinant CCKB Cholecystokinin Receptor Stable Cell Line NCL20120047CR GPR68/OGR1 Human Recombinant G-Protein Coupled Receptor 68 Stable Cell Line NCL20120048CR Class A orphan Human Recombinant Mas-related G Protein Coupled Receptor Member X2 Stable Cell Line MRGPRX2 NCL20120049CR Complement peptide receptor Human Recombinant Complement Component 5A Receptor 1 Stable Cell Line C5a/C5AR1 NCL20120050CR Human Recombinant Corticotropin Releasing Factor Receptor CRF1 Stable Cell Line CRF1/CRHR1 NCL20120051CR Corticotropin releasing factor receptor Human Recombinant Corticotropin Releasing Factor Receptor CRF2 Stable Cell Line CRF2 NCL20120052CR (Continuing…) 02 GPCR Stable Cell Line 09

10. Family Receptor Product Name Cat.No. D2 Human Recombinant D2 Dopamine Receptor Stable Cell Line NCL20120053CR D5 Human Recombinant D5 Dopamine Receptor Stable Cell Line NCL20120054CR Dopamine receptor D1/DRD1 Human Recombinant D1 Dopamine Receptor Stable Cell Line NCL20120055CR D2(Short) Human Recombinant D2 (Short) Dopamine Receptor Stable Cell Line NCL20120056CR ETA/EDNRA Human Recombinant Endothelin Receptor Type A Stable Cell Line NCL20120057CR Endothelin receptor ETB/EDNRB Human Recombinant Endothelin Receptor Type B Stable Cell Line NCL20120058CR Formylpeptide receptor FPR1 Human Recombinant Formylpeptide Receptor 1 Stable Cell Line NCL20120059CR FFA2 Human Recombinant Free Fatty Acid Receptor 2 Stable Cell Line NCL20120060CR Free fatty acid receptor GPR40/FFA1 Human Recombinant Free Fatty Acid Receptor 1 Stable Cell Line NCL20120061CR GALR1 Human Recombinant GAL1 Galanin Receptor Stable Cell Line NCL20120062CR Galanin receptor GALR2 Human Recombinant GAL2 Galanin Receptor Stable Cell Line NCL20120063CR Human Recombinant Growth Hormone Releasing Hormone Receptor Stable Cell Line GHRHR NCL20120064CR GLP1R Human Recombinant Glucagon-like Peptide 1 Receptor Stable Cell Line NCL20120065CR Glucagon receptor GLP2R Human Recombinant Glucagon-like Peptide 2 Receptor Stable Cell Line NCL20120066CR GCGR Human Recombinant Glucagon Receptor Stable Cell Line NCL20120067CR Human Recombinant Gastric Inhibitory Polypeptide Receptor Stable Cell Line GIPR NCL20120069CR Gonadotropin releasing hormone receptor Human Recombinant Gonadotropin-releasing Hormone Receptor Stable Cell Line GNRHR NCL20120070CR Growth hormone releasing peptides receptor Human Recombinant Growth Hormone Secretagogue Receptor Stable Cell Line GHSR NCL20120071CR H1/HRH1 Human Recombinant H1 Histamine Receptor Stable Cell Line NCL20120072CR H2/HRH2 Human Recombinant H2 Histamine Receptor Stable Cell Line NCL20120073CR Histamine receptor H3/HRH3 Human Recombinant H3 Histamine Receptor Stable Cell Line NCL20120074CR H4/HRH4 Human Recombinant H4 Histamine Receptor Stable Cell Line NCL20120075CR (Continuing…) 02 GPCR Stable Cell Line 10

11. Family Receptor Product Name Cat.No. Hydroxycarboxylic acid receptor GPR109A Human Recombinant Niacin Receptor 1 Stable Cell Line NCL20120076CR Kisspeptin receptor KiSS1/GPR54 Human Recombinant KiSS1-derived Peptide Receptor Stable Cell Line NCL20120077CR Human Recombinant Formyl Peptide Receptor Like-1 Receptor Stable Cell Line FPRL1 NCL20120078CR Leukotriene receptor CysLT1 Human Recombinant Cysteinyl Leukotriene Receptor 1 Stable Cell Line NCL20120079CR LTB4R Human Recombinant Leukotriene B4 Receptor Stable Cell Line NCL20120080CR Lysophospholipid receptor EDG4/LAP2 Human Recombinant EDG4 Lysophospholipid Receptor Stable Cell Line NCL20120081CR Human Recombinant Melanin-concentrating Hormone Receptor 1 Stable Cell Line MCH1/GPR24 NCL20120082CR Melanin concentrating hormone receptor MCH2/GPR145 Human Recombinant Melanin-concentrating Hormone Receptor 2 Stable Cell Line NCL20120083CR MC2 Human Recombinant MC2 Melanocortin Receptor Stable Cell Line NCL20120084CR MC3 Human Recombinant MC3 Melanocortin Receptor Stable Cell Line NCL20120085CR Melanocortin receptor MC4 Human Recombinant MC4 Melanocortin Receptor Stable Cell Line NCL20120086CR MC5 Human Recombinant MC5 Melanocortin Receptor Stable Cell Line NCL20120087CR MTNR1A Human Recombinant Melatonin MT1 Receptor Stable Cell Line NCL20120088CR Melatonin receptor MT2/MTNR1B Human Recombinant Melatonin MT2 Receptor Stable Cell Line NCL20120089CR Metabotropic glutamate receptor mGlu2/GRM2 Human Recombinant Metabotropic Glutamate Receptor 2 Stable Cell Line NCL20120090CR Motilin receptor MTLR/MLNR Human Recombinant Motilin Receptor Stable Cell Line NCL20120091CR NMU2 Human Recombinant Neuromedin U Receptor 2 Stable Cell Line NCL20120092CR Neuromedin receptor NMU1/GPR66 Human Recombinant Neuromedin U Receptor 1 Stable Cell Line NCL20120093CR NPS1b Human Recombinant Neuropeptide S Receptor Isoform B Stable Cell Line NCL20120094CR Neuropeptide S receptor NPSR1 Human Recombinant Neuropeptide S Receptor Isoform A Stable Cell Line NCL20120095CR NPY2 Human Recombinant Neuropeptide Y Receptor Y2 Stable Cell Line NCL20120096CR Neuropeptide Y receptor NPY4R Human Recombinant Neuropeptide Y Receptor Y4 Stable Cell Line NCL20120097CR (Continuing…) 02 GPCR Stable Cell Line 11

12. Family Receptor Product Name Cat.No. Neurotensin receptor NTS1/NT1 Human Recombinant NTS1 Neurotensin Receptor Stable Cell Line NCL20120098CR OPRD1 Human Recombinant δ-Opioid Receptor OPRD1 Stable Cell Line NCL20120099CR OPRK1 Human Recombinant κ-Opioid Receptor OPRK1 Stable Cell Line NCL20120100CR Opioid & opioid-like receptor OPRM1 Human Recombinant μ-Opioid Receptor OPRM1 Stable Cell Line NCL20120101CR OPRL1 Human Recombinant Opiate Receptor-like 1 Stable Cell Line NCL20120102CR OX1 Human Recombinant OX1 Orexin Receptor Stable Cell Line NCL20120103CR Orexin receptor OX2 Human Recombinant OX2 Orexin Receptor Stable Cell Line NCL20120104CR P2Y receptor P2Y1 Human Recombinant P2Y1 Purinergic Receptor Stable Cell Line NCL20120105CR P3Y receptor P2Y2 Human Recombinant P2Y2 Purinergic Receptor Stable Cell Line NCL20120106CR P4Y receptor P2Y4 Human Recombinant P2Y4 Purinergic Receptor Stable Cell Line NCL20120107CR P5Y receptor P2Y6 Human Recombinant P2Y6 Purinergic Receptor Stable Cell Line NCL20120108CR P6Y receptor P2Y11 Human Recombinant P2Y11 Purinergic Receptor Stable Cell Line NCL20120109CR P7Y receptor P2Y12 Human Recombinant P2Y12 Purinergic Receptor Stable Cell Line NCL20120110CR Parathyroid hormone receptor PTH1/PTHR1 Human Recombinant Parathyroid Hormone Receptor 1 Stable Cell Line NCL20120111CR Peptide P518 receptor QRFP/GPR103 Human Recombinant G-Protein Coupled Receptor 103 Stable Cell Line NCL20120112CR Platelet activating factor receptor PAFR/PTAFR Human Recombinant Platelet Activating Factor Receptor Stable Cell Line NCL20120113CR Prolactin releasing peptide receptor Human Recombinant Prolactin-releasing Peptide Receptor Stable Cell Line PRLHR NCL20120114CR DP2 Human Recombinant Prostanoid Receptor DP2 Stable Cell Line NCL20120115CR EP1/PTGER1 Human Recombinant EP1 Prostanoid Receptor Stable Cell Line NCL20120116CR EP2/PTGER2 Human Recombinant EP2 Prostanoid Receptor Stable Cell Line NCL20120117CR Prostanoid receptor FP/PTGFR Human Recombinant FP Prostanoid Receptor Stable Cell Line NCL20120118CR IP1/PTGIR Human Recombinant Prostanoid IP1 Receptor Stable Cell Line NCL20120119CR TP/TBXA2R Human Recombinant TP Prostanoid Receptor Stable Cell Line NCL20120120CR PTGDR/DP1 Human Recombinant Prostanoid Receptor DP1 Stable Cell Line NCL20120121CR (Continuing…) 02 GPCR Stable Cell Line 12

13. Family Receptor Product Name Cat.No. Prostanoid receptor DP2 Human Recombinant Prostanoid Receptor DP2 Stable Cell Line NCL20120115CR Human Recombinant PAR2 Proteinase-activated Receptor Stable Cell Line PAR2 NCL20120122CR Proteinase-activated receptor Human Recombinant PAR4 Proteinase-activated Receptor Stable Cell Line PAR4/F2RL3 NCL20120123CR SST1 Human Recombinant SST1 Somatostatin Receptor Stable Cell Line NCL20120124CR SST2/SSTR2 Human Recombinant SST2 Somatostatin Receptor Stable Cell Line NCL20120125CR Somatostatin receptor SST3 Human Recombinant SST3 Somatostatin Receptor Stable Cell Line NCL20120126CR SST4/SSTR4 Human Recombinant SST4 Somatostatin Receptor Stable Cell Line NCL20120127CR SST5/SSTR5 Human Recombinant SST5 Somatostatin Receptor Stable Cell Line NCL20120128CR NK1/TACR1 Human Recombinant NK1 Tachykinin Receptor Stable Cell Line NCL20120129CR Tachykinin receptor NK2/TACR2 Human Recombinant NK2 Tachykinin Receptor Stable Cell Line NCL20120130CR NK3/TACR3 Human Recombinant NK3 Tachykinin Receptor Stable Cell Line NCL20120131CR Thyrotropin releasing hormone receptor Human Recombinant TRH1 Thyrotropin-releasing Hormone Receptor Stable Cell Line TRH1/TRHR NCL20120132CR Urotensin receptor UTR2 Human Recombinant Urotensin 2 Receptor Stable Cell Line NCL20120133CR OT/OXTR Human Recombinant Oxytocin Receptor Stable Cell Line NCL20120134CR Vasopressin and oxytocin receptor V1B Human Recombinant V1B Vasopressin Receptor Stable Cell Line NCL20120135CR V2 Human Recombinant V2 Vasopressin Receptor Stable Cell Line NCL20120136CR Human Recombinant Vasoactive Intestinal Polypeptide Receptor Stable Cell Line PAC1 NCL20120137CR VIP and PACAP receptor VPAC2 Human Recombinant VPAC2 Receptor Stable Cell Line NCL20120138CR VPAC1/VIP1 Human Recombinant VPAC1 Receptor Stable Cell Line NCL20120139CR Control HEK293/Gα15 Stable Cell Line NCL20120140CR Control Human Recombinant Gα15 Stable Cell Line NCL20120141CR Human Recombinant Gqi5 Stable Cell Line NCL20120142CR 02 GPCR Stable Cell Line 13

14. Sensory neurons, also known as afferent neurons, are the nerve cells activated by sensory input from the environment. Different types of sensory neurons have different sensory receptors that respond to different kinds of stimuli. Function Type Smell Olfactory Sensory Neuron Taste Taste Receptor Cell Vision Photoreceptor Cell External Stimuli Auditory Hair Cell Temperature Thermoreceptor Cell Mechanical Force Mechanoreceptor Cell Blood Glomus Cell Internal Stimuli Potentially Damaging Stimuli Nociceptor Cell 03 Sensory Neuron Culture 14

15. Creative Biolabs' specialty media allows neuroscience researchers to drastically accelerate manufacture of neurons from human pluripotent stem cells. NeuSenso™ Sensory Neurons NeuSenso™ Sensory Neurons Maturation Medium NeuSenso™ hPSC Differentiation Medium Cryopreserved hiPSC- derived highly characterized sensory neurons manufactured for off-the- shelf use. Human pluripotent stem cell differentiation medium for the rapid manufacture of dorsal root ganglia-like sensory neurons. For the maturation of Sensory Neurons into electrophysiological responsive neurons. Fast.Sensory Neurons need only one week of maturation before firing action potentials. Consistent.Sensory Neurons have a consistent lot-to-lot phenotype and post-thaw recovery. Fast. Generates sensory neurons in an easy-to-use monolayer protocol in only seven days. Functional.Sensory Neurons possess potassium, calcium, and sodium currents, including NaV 1.7. Simple. Sensory Neurons are pure and maturation- ready with no need for mitomycin treatment. Efficient. Manufactures up to 12 million sensory neurons with 90% purity for BRN3A, ISL1, and PERIPHERIN. 03 Sensory Neuron Culture 15

16. Intracellular Ca2+, as one of the most important second messengers, is central to neuronal signaling. Abnormalities in Ca2+ signaling have severe pathological consequences and can result in neurodegeneration, disorders of the central nervous, etc. Genetically encoded calcium indicators (GECIs) are widely used in the studies of neuron. The GECI binds to calcium ions intracellular calcium concentration, enables detection of in vivo neuronal firing activity. Various GECIs have been developed and can be chosen for diverse purposes. and emits fluorescence visualizing https:///files/Articles/127www.fro ntiersin.org475/fphar HTML/image_m/fphar g001.jpg Overview of GPCR Signal Detection by GCaMP Sensor [Reference: Partridge, J. G. (2015). Utilizing GCaMP transgenic mice to monitor endogenous Gq/11- coupled receptors. Frontiers in pharmacology, 6, 42.] 04 Calcium&Glutamate Signaling 16

17. Glutamate is a nonessential amino acid, a major bioenergetic substrate for proliferating normal and neoplastic cells, and an excitatory neurotransmitter that is actively involved in biosynthetic, bioenergetic, metabolic, and oncogenic signaling pathways. Glutamate signaling activates a family of receptors consisting of metabotropic glutamate receptors (mGluRs) and ionotropic glutamate receptors (iGluRs), both of which have been implicated in chronic disabling brain disorders such as Schizophrenia and neurodegenerative diseases like Alzheimer's, Parkinson's, and multiple sclerosis. GluR Signaling at the Synapse [Reference: Reiner, A., & Levitz, J. (2018). Glutamatergic Signaling in the Central Nervous System: Ionotropic and Metabotropic Receptors in Concert. Neuron, 98(6), 1080–1098. doi:10.1016/j.neuron.2018.05.018] Genetically encoded fluorescent glutamate indicators (iGluSnFRs) enable neurotransmitter release and diffusion to be visualized in intact tissue. 04 Calcium&Glutamate Signaling 17

18. Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR Biosensors are genetically encoded fluorescent proteins (FP) attached to an additional protein sequence which makes them sensitive to small biomolecules (e.g., Ca2+). Adeno-associated virus (AAV) Biosensor products come as ready-to-use AAV virus. The viruses encode your chosen biosensor, either calcium or neurotransmitter sensors and are ready for in vivo injection. Creative Biolabs offers a range of Calcium or Neurotransmitter Biosensor products that come ready-to-use, with a choice of promoter and the ability to include the Cre inducible (FLEX-ON) expression. We have packaged these indicators into the most commonly used AAV serotypes (AAV8 and AAV9). Our Products Our collection of over 2,000 AAV products is made up of various genetic elements, packaged into different serotypes in multiple combinations. The table below shows the popular options available from us. Please contact us if you need a different serotype. Available Options ALB, aMHC, c-Fos, CAG, CaMKIIa, CK0.4, CK1.3, CMV, cTnT, EF1a, EFFS, GFAP, HCRApoE, MBP, Promoters MCK, MeCP2, NSE, PDX1, PGK, Rpe65, SST, Syn, TBG, UBC GFP, mCherry, RFP, LacZ, Luciferase Reporters DIO-GFP, DIO-mCherry, DIO-RFP, DIO-LacZ Inducible reporters Cre, DIO, tTA Inducible systems Cas9, gRNA scaffold CRISPR/Cas9 AAV1, AAV2, AAV5, AAV6, AAV7, AAV8, AAV9, AAV-DJ Serotypes Unsure which serotype to use? If you are unsure which serotype will work best with your application, try our AAV GFP Testing Kit (Cat. NEK-2101- ZP27). This kit contains CMV-GFP in AAV1, AAV2, AAV5. AAV6. AAV8, AAV9, and AAV-DJ serotypes so you can test the efficiency on different cell types. 04 Calcium&Glutamate Signaling 18

19. Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR 1. Deciding which biosensor is most suitable for your research goal (calcium or glutamate， color, kinetics, SNR). 2. Considering whether you would like to include FLEX. 3. Choosing a promoter (universal promoter-CAG, or tissue specific neuron specific-synapsin promoter-Syn). 4. Choosing the AAV vector serotype (AAV8 or AAV9). Other serotypes are available upon request. Biosensor Promoter Description AAV type Catalogue No. iGluSnFR CMV AAV8-CMV-iGluSnFR AAV8 NTC2101010CR Rapid detection of glutamate, improved SNR; direct visualization of synaptic release (as opposed to Ca2+ imaging) Glutamate CMV AAV9-CMV-iGluSnFR AAV9 NTC2101011CR CaMPARI CAG AAV8-CAG-FLEX-CaMPARI AAV8 NTC2101001CR Syn AAV8-Syn-CaMPARI AAV8 NTC2101013CR Syn AAV8-Syn-FLEX-CaMPARI AAV8 NTC2101014CR High throughput calcium assays with cultured cells CAG AAV9-CAG-FLEX-CaMPARI AAV9 NTC2101029CR Syn AAV9-Syn-CaMPARI AAV9 NTC2101043CR Calcium Syn AAV9-Syn-FLEX-CaMPARI AAV9 NTC2101044CR CAG AAV8-CAG-GCaMP3 AAV8 NTC2101009CR GCaMP3: Green indicator CAG AAV9-CAG-GCaMP3 AAV9 NTC2101041CR Syn AAV8-pRSET.GCaMP5G(7.35) AAV8 NTC2101012CR GCaMP5: Green indicator CAG AAV9-pRSET.GCaMP5G(7.35) AAV9 NTC2101042CR (Continuing…) For more information, please visit our website: AAV Neural Tracers. 04 Calcium&Glutamate Signaling 19

20. Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR Biosensor Promoter Description AAV type Catalogue No. CAG AAV8-CAG-FLEX-GCaMP6s-WPRE AAV8 NTC2101004CR Syn AAV8-Syn-FLEX-GCaMP6s-WPRE AAV8 NTC2101017CR GCaMP6s: Improved SNR, slower kinetics; Green indicator Syn AAV8-Syn-GCaMP6s-WPRE AAV8 NTC2101024CR CAG AAV9-CAG-FLEX-GCaMP6s-WPRE AAV9 NTC2101032CR Syn AAV9-Syn-FLEX-GCaMP6s-WPR AAV9 NTC2101047CR Syn AAV9-Syn-GCaMP6s-WPRE AAV9 NTC2101058CR CAG AAV8-CAG-FLEX-GCaMP6m-WPRE AAV8 NTC2101003CR Syn AAV8-Syn-FLEX-GCaMP6m-WPRE AAV8 NTC2101016CR GCaMP6m: Improved SNR, intermediate kinetics; Green indicator Syn AAV8-Syn-GCaMP6m-WPRE AAV8 NTC2101023CR Calcium CAG AAV9-CAG-FLEX-GCaMP6m-WPRE AAV9 NTC2101031CR Syn AAV9-Syn-FLEX-GCaMP6m-WPRE AAV9 NTC2101046CR Syn AAV9-Syn-GCaMP6m-WPRE AAV9 NTC2101057CR CAG AAV8-CAG-FLEX-GCaMP6f-WPRE AAV8 NTC2101002CR Syn AAV8-Syn-FLEX-GCaMP6f-WPRE AAV8 NTC2101015CR GCaMP6f: Improved SNR, faster kinetics; Green indicator Syn AAV8-Syn-GCaMP6f-WPRE AAV8 NTC2101022CR CAG AAV9-CAG-FLEX-GCaMP6f-WPRE AAV9 NTC2101030CR Syn AAV9-Syn-FLEX-GCaMP6f-WPRE AAV9 NTC2101045CR Syn AAV9-Syn-GCaMP6f-WPRE AAV9 NTC2101056CR (Continuing…) For more information, please visit our website: AAV Neural Tracers. 04 Calcium&Glutamate Signaling 20

21. Calcium Indicators - CaMPARI, GCaMP, RCaMP, jRGECO1 | Glutamate Indicators - iGluSnFR Biosensor Promoter Description AAV type Catalogue No. CAG AAV8-CAG-FLEX-NES-jRCaMP1a-WPRE AAV8 NTC2101005CR Syn AAV8-Syn-FLEX-NES-jRCaMP1a-WPRE AAV8 NTC2101018CR jRCaMP1a: Red, improved SNR, slower kinetics, bright Syn AAV8-Syn-NES-jRCaMP1a-WPRE AAV8 NTC2101025CR CAG AAV9-CAG-FLEX-NES-jRCaMP1a-WPRE AAV9 NTC2101037CR Syn AAV9-Syn-FLEX-NES-jRCaMP1a-WPRE AAV9 NTC2101052CR Syn AAV9-Syn-NES-jRCaMP1a-WPRE AAV9 NTC2101063CR CAG AAV8-CAG-FLEX-NES-jRCaMP1b-WPRE AAV8 NTC2101006CR Syn AAV8-Syn-FLEX-NES-jRCaMP1b-WPRE AAV8 NTC2101019CR jRCaMP1b: Red, improved SNR, improved dynamic range, bright Syn AAV8-Syn-NES-jRCaMP1b-WPRE AAV8 NTC2101026CR CAG AAV9-CAG-FLEX-NES-jRCaMP1b-WPRE AAV9 NTC2101038CR Syn AAV9-Syn-FLEX-NES-jRCaMP1b-WPRE AAV9 NTC2101053CR Syn AAV9-Syn-NES-jRCaMP1b-WPRE AAV9 NTC2101064CR Calcium CAG AAV8-CAG-FLEX-NES-jRGECO1a-WPRE AAV8 NTC2101007CR Syn AAV8-Syn-FLEX-NES-jRGECO1a-WPRE AAV8 NTC2101020CR Syn AAV8-Syn-NES-jRGECO1a-WPRE AAV8 NTC2101027CR jRGECO1a: Red, improved SNR CAG AAV9-CAG-FLEX-NES-jRGECO1a-WPRE AAV9 NTC2101039CR Syn AAV9-Syn-FLEX-NES-jRGECO1a-WPRE AAV9 NTC2101054CR Syn AAV9-Syn-NES-jRGECO1a-WPRE AAV9 NTC2101065CR CAG AAV8-CAG-FLEX-NES-jRGECO1b-WPRE AAV8 NTC2101008CR Syn AAV8-Syn-FLEX-NES-jRGECO1b-WPRE AAV8 NTC2101021CR Syn AAV8-Syn-NES-jRGECO1b-WPRE AAV8 NTC2101028CR jRGECO1b: Red, improved SNR CAG AAV9-CAG-FLEX-NES-jRGECO1b-WPRE AAV9 NTC2101040CR Syn AAV9-Syn-FLEX-NES-jRGECO1b-WPRE AAV9 NTC2101055CR Syn AAV9-Syn-NES-jRGECO1b-WPRE AAV9 NTC2101066CR For more information, please visit our website: AAV Neural Tracers. 04 Calcium&Glutamate Signaling 21

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