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Lesson 1-8

Lesson 1-8. Laboratory Math and Reagent Preparation. Overview. Laboratory reagents Chemical purity Laboratory reagent water Pipet use Safety Quality assessment Formulas for preparing solutions Making dilutions. Terms. Solution Solvent Solute Dilution. Common Laboratory Reagents.

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Lesson 1-8

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  1. Lesson 1-8 Laboratory Math and Reagent Preparation

  2. Overview • Laboratory reagents • Chemical purity • Laboratory reagent water • Pipet use • Safety • Quality assessment • Formulas for preparing solutions • Making dilutions

  3. Terms • Solution • Solvent • Solute • Dilution

  4. Common Laboratory Reagents • Disinfectants • Dilute acids • Stock solutions • Working solutions • Controls

  5. Chemical Purity • Use reagent or analytical grade • Ultrapure sometimes required • Lower grades not used include: • Technical grade • Practical • Commercial

  6. Laboratory Reagent Water • Types specified by CLSI • Made by further treatment of • Distilled water • Deionized water • Reverse osmosis water

  7. Laboratory Reagent Water • CLSI – grades of water • Clinical Laboratory Reagent Water (CLRW) • Special reagent water • Instrument feed water • Autoclave and wash water • Commercially bottled purified water • See Table 1-34

  8. Pipets • TD or TC • Volumetric pipets • Serological/graduated pipets

  9. Pipetting Aids • Manual • Battery or electrical

  10. Micropipetters • Advantages • Disposable tips • Adjustable volumes • Improved accuracy • Improved reproducibility

  11. Micropipetters

  12. Safety • Chemical hazards • Consult MSDS • Biological hazards • biological controls • Physical hazards

  13. Quality Assessment • Correct formulas • Correct calculations • Pipet techniques • Selection of labware • Reagent storage • Expiration dates • Test new reagents

  14. Preparing Dilutions • Using proportion to make a dilution

  15. Preparing Dilutions • Using a concentrate to make a dilute solution

  16. Preparing Dilutions • Using ratios to prepare dilutions

  17. Preparing Percentage Solutions • Weight/volume

  18. Preparing Percentage Solutions • Volume/volume • Weight/weight

  19. Preparing Molar Solutions • Molar (M) solution = moles solute per liter solution (mol/L) • Formula weight • Mole

  20. Serial Dilutions • Two-fold dilution series

  21. Compound Dilutions

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