1 / 8

PCR

PCR. By: Cody Alveraz Ted Dobbert Morgan Pettit . What is PCR?. PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences. It was developed in 1983 by Kary Mullis.

callum-shelton
Download Presentation

PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PCR By: Cody Alveraz Ted Dobbert Morgan Pettit

  2. What is PCR? • PCR is a polymerase chain reaction that copies pieces of DNA across multiple orders of magnitude, creating thousands to millions of copies of DNA sequences. • It was developed in 1983 by Kary Mullis.

  3. Procedures of PCR • Denaturation: At 201.2 F degrees the double stranded DNA melts and opens into 2 pieces of single-stranded DNA. • Annealing: At medium temperatures, at around 54 degrees Celsius, the primers pair up with the single-stranded “template”. On the small length of double-stranded DNA, the polymerase attaches and starts copying the template.

  4. Procedures of PCR (cont.) • Extension: AT 72 degrees Celsius, where the polymerase works best, and DNA building blocks complementary to the template are coupled to the primer, making a double-stranded DNA molecule. • With one cycle a segment of double-stranded DNA template is amplified into two separate pieces of double stranded DNA.

  5. What is good about PCR? • PCR is good technique because its not expensive, rapid and a good way to get millions of copies of DNA.

  6. PCR Process Diagram

  7. PCR Machine

  8. Links: • http://www.medicinenet.com/pcr_polymerase_chain_reaction/article.htm • http://www.youtube.com/watch?v=eEcy9k_KsDI

More Related