1 / 9

PCR

PCR. PCR AMPLIFICATION. PCR APPLICATIONS. MOLECULAR BIOLOGY ENVIRONMENTAL SCIENCE FORENSIC/MEDICAL SCIENCE BIOTECHNOLOGY GENETICS GENE CLONING MICROBIOLOGY. DNA STRUCTURE. SUGAR-PHOSPHATE BACKBONE A=ADENINE T=THYMINE C=CYTOSINE G=GUANINE. PCR REQUIREMENTS. DNA PRIMERS

elina
Download Presentation

PCR

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. PCR

  2. PCR AMPLIFICATION

  3. PCR APPLICATIONS • MOLECULAR BIOLOGY • ENVIRONMENTAL SCIENCE • FORENSIC/MEDICAL SCIENCE • BIOTECHNOLOGY • GENETICS • GENE CLONING • MICROBIOLOGY

  4. DNA STRUCTURE • SUGAR-PHOSPHATE BACKBONE • A=ADENINE • T=THYMINE • C=CYTOSINE • G=GUANINE

  5. PCR REQUIREMENTS • DNA • PRIMERS • dATP, dTTP, dCTP, dGTP • POLYMERASES

  6. RAPD PCR • RANDOM • AMPLIFIED • POLYMORPHIC • DNA

  7. RAPD APPLICATIONS • DNA FINGERPRINTING • ANALYSIS OF ANCIENT DNA FROM FOSSILS • MAPPING THE HUMAN GENOME • FINGERPRINTING UNKNOWN MICROORGANISMS

  8. RAPD PRIMERS • 10 NUCLEOTIDES LONG • ARBITRARY SEQUENCE • BINDS AT MULTIPLE SEQUENCES ALONG DNA • NON-SPECIFIC BINDING • ACCURACY REQUIRES AT LEAST 10 PRIMERS

More Related