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Phenotype R S R S S R R S

calvin
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Phenotype R S R S S R R S

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  1. 1. You are mapping both ends of a large genomic clone that was selected using a DNA marker that cosegregated perfectly with the locus in 251 test cross progeny. You map the ends on another 1000 test cross progeny (below). What can you conclude about the clone from this data? What would you next do with this clone? Rp1 rp1 Parent Parent Test cross progeny Phenotype R S R S S R R S Left end Right end # progeny 489 478 14 16 1 2

  2. 2. Same problem as in problem 1, but different data. Rp1 rp1 Parent Parent Test cross progeny Phenotype R S R S S R S R Left end Right end # progeny 506 487 3 2 1 1

  3. 3) You mutagenize a line that is homozygous for the rust resistance gene R1 with the intent of either finding mutants in R1 or finding another locus required for R1 mediated resistance (you will call the second locus Rr1 for Required for R1). You screen a bunch of M2 progeny from the mutagenized R1 line and find a single family that is all highly susceptible. You cross this susceptible line to the R1 line and to another susceptible cultivar (r1/r1) and. Your F1 with the R1 line is resistant and the F2 from this cross segregates 3 resistant: 1 susceptible. The F1 from the cross with the susceptible line is susceptible and the F2 progeny from this cross is all susceptible. Is the mutant you selected from a mutation in the Rr1 locus or the R1 locus? 4) You find a second mutant susceptible family from the above mutagenized population. You cross this second susceptible line to the R1 line and to another susceptible cultivar (r1/r1) and. Your F1 with the R1 line is resistant and the F2 from this cross segregates 3 resistant: 1 susceptible. The F1 from the cross with the susceptible line is resistant and the F2 progeny from this cross segregate roughly 9 resistant to 7 susceptible. Is this second mutant you selected from a mutation in the Rr1 locus or the R1 locus?

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