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Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student

Oral presentation CREATE Gj ø vik. Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student. There will be:. Short introduction to fluorescence Few applications Something about bioanalytics Coloring or whitening merchandise Arts

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Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student

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  1. Oral presentation CREATE Gjøvik Fluorescence : One phenomenon, many applications Jussi Kinnunen, MSc (Physics), PhD student

  2. There will be: • Short introduction to fluorescence • Few applications • Something about bioanalytics • Coloring or whitening merchandise • Arts • Measurements and data analysis • by using two monochromator –method Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  3. What is fluorescence?

  4. For me it is fancy colours Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  5. Light interaction with matter When light comes When light goes Fluorescence emission Lifetime about 10-8(10-10) s Lower energy photons than the one(s) absorbed Phosphorescence Lifetime >10-8 s Lower energy photons than the one(s) absorbed Scattering Energy if the photon is close to the incident one(s) • Absorption • Time about 10-15 s • Scattering • Time about 0 s A F P Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  6. Applications: Bioanalytics

  7. Fast detection of small quantities of anything • Idea: • Choose a receptor for the target • Attach label (reporter) to the receptor • Illuminate and see • Where is your target • e.g. cancer cells • Was there any of your target • e.g. DNA sequencing Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  8. Applications: Industry

  9. Making things white Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  10. Applications: Art

  11. Masterpiece (Miera, 1950’s), white light and UVPhoto courtesy of Nick Padalino Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  12. Color measurements of fluorescence

  13. Two monochromator method Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  14. Measured data • Donaldson matrix is calculated from the measured data • The reflectance • On diagonal • The fluorescence • Off diagonal Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  15. Simulations

  16. Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  17. Simulation with the scientific brown Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  18. Lights • Simulations under • Tungsten light (green) • Daylight simulator (blue) • White led (red) • Fluorescent light (cyan) Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  19. Under “smooth” light sources Halogen light Daylight simulator The black curve is measured under polycromatic light fluorescence total reflectance Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  20. Under “peaky” light sources Led light Fluorescent light The black curve is measured under polycromatic light total fluorescence reflectance Fluorescence : One phenomenon, many applications / Jussi Kinnunen

  21. Thank you. www.uef.fi

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