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Principles of Fluorescence Spectroscopy. Chemistry Department XMU. Chapter Six. Methods of Fluorometry. Methods of fluorometry . 6.1 Introduction 6.2 Conventional Fluorometry 6.3 Synchronous Fluorescence Scan 6.4 3D fluorescence spectroscopy
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XMUGXQ PFS0601 Principles of Fluorescence Spectroscopy Chemistry Department XMU
XMUGXQ PFS0601 Chapter Six Methods of Fluorometry
XMUGXQ PFS0601 Methods of fluorometry 6.1 Introduction 6.2 Conventional Fluorometry 6.3 Synchronous Fluorescence Scan 6.4 3D fluorescence spectroscopy 6.5 Derivative fluorescence spectroscopy
XMUGXQ PFS0601 I0 I0 I0 t t t 6.1 Introduction Light source Models Steady-state fluorescence measurement Time-domain fluorescence measurement Time resolved fluorescence Frequency-domain fluorescence measurement
XMUGXQ PFS0601 em F F 3 1 2 4 6.3 Synchronous Fluorescence Scan SFS Conventional Fluorometry Excitation spectrum Scan ex at a certain em Emission spectra Scan em at a certain ex
XMUGXQ PFS0601 Synchronous fluorescence scan SFS Scan ex and em simultaneously models Constant-wavelength SFS Constant-energy SFS Variable-angle SFS
XMUGXQ PFS0601 Constant-wavelength SFS
XMUGXQ PFS0601 Narrowing spectral band & simplifyingemission spectrum
XMUGXQ PFS0601 Narrowing spectral band & simplifyingemission spectrum
XMUGXQ PFS0601 F SFS F CFS F em em Multi-component analysis
XMUGXQ PFS0601 Theory Where Ex is the excitation wave function at a given wavelength; Em is the emission wave function at a given wavelength; l is the thickness of the sample; K is the instrumental geometry and related parameters.
XMUGXQ PFS0601 F Choosing the Generally, the stoke’s shift
XMUGXQ PFS0601 Choosing the
XMUGXQ PFS0601 Choosing the
XMUGXQ PFS0601 PAHs Constant-energy SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Variable-angle SFS
XMUGXQ PFS0601 Reference Trends in analytical chemistry, 21(12): 787-798, 2002
XMUGXQ PFS0601 3 dimensional fluorescence spectroscopy
XMUGXQ PFS0601 Contour 3D spectra
XMUGXQ PFS0601 Finger printing
XMUGXQ PFS0601 Volume integral
XMUGXQ PFS0601 芘 Derivative fluorescence
XMUGXQ PFS0601 Derivative fluorescence 峰零法 峰距法 切线法
XMUGXQ PFS0601 b a application
XMUGXQ PFS0601 application
XMUGXQ PFS0601 application
XMUGXQ PFS0601 application Finger printing