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This supplementary method involves running a gel of human single muscle fibers from a healthy older subject to identify Myosin Heavy Chain (MyHC) using SDS-PAGE. Lanes B, E, and F represent type I fibers, while Lanes A and D represent type IIa fibers. Lane C is empty.
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Supplementary Methods A B C D E F Representative SDS-PAGE gel of human single muscle fibers for MyHC identification from a healthy older subject. Lanes B, E and F correspond to type I fibers. Lanes A and D correspond to type IIa fibers. Lane C is empty.