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Genetic Engineering/Recombinant DNA. What is it?. Involves the transfer of genetic material from one organism to another. Results in the formation of Recombinant DNA . Human genes that control the synthesis of insulin and growth hormone have been inserted into bacteria.
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What is it? • Involves the transfer of genetic material from one organism to another. • Results in the formation of Recombinant DNA. • Human genes that control the synthesis of insulin and growth hormone have been inserted into bacteria. • Bacterial cells are then able to synthesize (make) certain substance needed by humans!
Steps: • Cut out donor DNA with restriction enzyme • Cut open plasmid with restriction enzyme • Insert donor DNA into open plasmid • Add ligase to bond donor DNA and plasmid • Obtain synthesized protein https://www.youtube.com/watch?v=TpmNfv1jKuA