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Gene Order Phylogeny. Tandy Warnow The Program in Evolutionary Dynamics, Harvard University The University of Texas at Austin. Cyber-Infrastructure for Phylogenetic RESearch (http://www.phylo.org)
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Gene Order Phylogeny Tandy Warnow The Program in Evolutionary Dynamics, Harvard University The University of Texas at Austin
Cyber-Infrastructure for Phylogenetic RESearch (http://www.phylo.org) • Main research: Large-scale phylogenetics, reticulate evolution, gene order phylogeny, complex simulations, and databases • Funded by $11.6M ITR Grant from NSF • 40 biologists, computer scientists, and mathematicians collaborating on the project
University of New Mexico Bernard Moret David Bader Tiffani Williams UCSD/SDSC Fran Berman Alex Borchers David Stockwell Phil Bourne John Huelsenbeck Dana Jermanis Mark Miller Michael Alfaro Tracy Zhao University of Connecticut Paul O Lewis University of Pennsylvania Junhyong Kim Sampath Kannan UT Austin Tandy Warnow David M. Hillis Warren Hunt Robert Jansen Randy Linder Lauren Meyers Daniel Miranker Usman Roshan Luay Nakhleh University of Arizona David R. Maddison University of British Columbia Wayne Maddison North Carolina State University Spencer Muse American Museum of Natural History Ward C. Wheeler UC Berkeley Satish Rao Joseph M. Hellerstein Richard M Karp Brent Mishler Elchanan Mossel Eugene W. Myers Christos M. Papadimitriou Stuart J. Russell SUNY Buffalo William Piel Florida State University David L. Swofford Mark Holder Yale Michael Donoghue Paul Turner Aventis Pharmaceuticals Lisa Vawter CIPRes Members
Limitations of DNA phylogenetics • Deep evolutionary histories may not be recoverable from DNA sequence phylogeny due to lack of specificity -- too much noise (homoplasy) and insufficient sequence length • The systematics community has looked to “rare genomic changes” for better sources of phylogenetic signal
A C A D X E Y B E Z W C F B D F Whole-Genome Phylogenetics
Genomes As Signed Permutations 1 –5 3 4 -2 -6or6 2 -4 –3 5 –1 etc.
1 2 3 –8 –7 –6 –5 -4 9 10 1 2 3 9 -8 –7 –6 –5 –4 10 1 2 3 9 4 5 6 7 8 10 Genomes Evolve by Rearrangements 1 2 3 4 5 6 7 8 9 10 • Inversion (Reversal) • Transposition • Inverted Transposition
Other types of events • Duplications, Insertions, and Deletions (changes gene content) • Fissions and Fusions (for genomes with more than one chromosome) These events change the number of copies of each gene in each genome (“unequal gene content”)
Genome Rearrangement Has A Huge State Space • DNA sequences : 4 states per site • Signed circular genomes with n genes: states, 1 site • Circular genomes (1 site) • with 37 genes (mitochondria): states • with 120 genes (chloroplasts): states
Why use gene orders? • “Rare genomic changes”: huge state space and relative infrequency of events (compared to site substitutions) could make the inference of deep evolution easier, or more accurate. • Our research shows this is true, but accurate analysis of gene order data is computationally very intensive!
Phylogeny reconstruction from gene orders • Distance-based reconstruction: estimate pairwise distances, and apply methods like Neighbor-Joining or Weighbor • “Maximum Parsimony”: find tree with the minimum length (inversions, transpositions, or other edit distances) • Maximum Likelihood: find tree and parameters of evolution most likely to generate the observed data
A A D D B B 3 3 Total length = 18 6 C C E F 4 2 Maximum Parsimony on Rearranged Genomes (MPRG) • The leaves are rearranged genomes. • Find the tree that minimizes the total number of rearrangement events (e.g., inversion phylogeny minimizes the number of inversions)
Optimization problems for gene order phylogeny • Breakpoint phylogeny: find the phylogeny which minimizes the total number of breakpoints (NP-hard, even to find the median of three genomes) • Inversion phylogeny: find the phylogeny which minimizes the sum of inversion distances on the edges (NP-hard, even to find the median of three genomes)
Inversion phylogenies • When the data are close to saturated, even the best distance-based analyses are insufficiently accurate. In these cases, our initial investigations suggest that the inversion phylogeny approach may be superior. • Problem: finding the best trees is enormously hard, since even the “point estimation” problem is hard (worse than estimating branch lengths in ML). Local optimum Tree length Global optimum Phylogenetic trees
Observations • For equal gene content, heuristics for the inversionphylogeny problem are extremely accurate, even under model conditions in which transpositions are dominant. • For unequal gene content, the parsimony style problems are too computationally intense -- but NJ (neighbor joining) with a new distance estimator (Moret et al. 2004) works extremely well.
Software • BPAnalysis (Sankoff): open source, restricted to the breakpoint phylogeny reconstruction • GRAPPA (Moret et al.): open source, restricted to single chromosome genomes, but can handle both equal and unequal gene content • MGR (Pevzner et al.): multiple chromosome, limited to equal gene content, performs well if the dataset is small (less than 10 genomes) • Bayesian analysis by Bret Larget (not yet released).
Merciera Wahlenbergia Tiodanus Legousia Asyneuma Trachelium Symphyandra Campanula Codonopsis Tobacco Adenophora Cyananthus The strict consensus of 24 trees, each with inversion length of 64. Finished within 40 minutes on a laptop using GRAPPA version 1.8 Platycodon
GRAPPA (Genome Rearrangement Analysis under Parsimony and other Phylogenetic Algorithms) http://www.cs.unm.edu/~moret/GRAPPA/ • Heuristics for maximum parsimony style problems for equal gene content • Fast polynomial time distance-based methods • Contributors: U. New Mexico,U. Texas at Austin, Universitá di Bologna, Italy • Freely available in source code at this site. • Project leader: Bernard Moret (UNM) (moret@cs.unm.edu)
Speeding up MP and ML: DCM3 Tandy Warnow Radcliffe Institute The University of Texas at Austin
Reconstructing the “Tree” of Life Handling large datasets: millions of species
Main research objectives • Determine the best current methods available for MP and ML, and then improve upon them • Focus on performance within one day, one week, or one month, on large real datasets (1K to 20K sequences for MP) • Final objective is hundreds of thousands (or millions) of sequences.
Initial results • Very large datasets are hard for both MP and ML, no matter what software is used • Suboptimal solutions to MP yield reasonable estimates of the optimal MP trees - but only if they are within .01% of optimal MP score • Improving upon techniques for searching treespace will yield improvements for both MP and ML
Datasets Obtained from various researchers and online databases • 1322 lsu rRNA of all organisms • 2000 Eukaryotic rRNA • 2594 rbcL DNA • 4583 Actinobacteria 16s rRNA • 6590 ssu rRNA of all Eukaryotes • 7180 three-domain rRNA • 7322 Firmicutes bacteria 16s rRNA • 8506 three-domain+2org rRNA • 11361 ssu rRNA of all Bacteria • 13921 Proteobacteria 16s rRNA
Problems with current techniques for MP Average MP scores above “optimal” of best methods at 24 hours across 10 datasets Best current techniques fail to reach 0.01% of optimal at the end of 24 hours, on large datasets
Problems with current techniques for MP The best current method (default TNT) fails to reach acceptable levels of accuracy (0.01% of “optimal”) within 24 hours on many large datasets -- evidence suggests that this level will not be reached for weeks or months (or more) of further analysis. Performance of TNT with time
Observations • The best methods cannot get acceptably good solutions within 24 hours on most of these large datasets. • Datasets of these sizes may need months (or years) of further analysis to reach reasonable solutions. • Apparent convergence can be misleading.
Observations • The best methods cannot get acceptably good solutions within 24 hours on most of these large datasets. • Datasets of these sizes may need months (or years) of further analysis to reach reasonable solutions. • Apparent convergence can be misleading.
Observations • The best methods cannot get acceptably good solutions within 24 hours on most of these large datasets. • Datasets of these sizes may need months (or years) of further analysis to reach reasonable solutions. • Apparent convergence can be misleading.
“Boosting” MP heuristics • DCMs “boost” the performance of phylogeny reconstruction methods. DCM Base method M DCM-M
Iterative-DCM3 T DCM3 Base method T’
New DCMs • DCM3 • Compute subproblems using DCM3 decomposition • Apply base method to each subproblem to yield subtrees • Merge subtrees using the Strict Consensus Merger technique • Randomly refine to make it binary • Recursive-DCM3 • Iterative DCM3 • Compute a DCM3 tree • Perform local search and go to step 1 • Recursive-Iterative DCM3
Performance Study • How well do these “boosted” versions of the best MP heuristics perform, compared to the best MP heuristics? • We examine performance with respect to “optimal” MP scores (best found so far, using any method) for a number of very large datasets, over 24 hours. • The benchmark MP heuristic is the default TNT.
Datasets Obtained from various researchers and online databases • 1322 lsu rRNA of all organisms • 2000 Eukaryotic rRNA • 2594 rbcL DNA • 4583 Actinobacteria 16s rRNA • 6590 ssu rRNA of all Eukaryotes • 7180 three-domain rRNA • 7322 Firmicutes bacteria 16s rRNA • 8506 three-domain+2org rRNA • 11361 ssu rRNA of all Bacteria • 13921 Proteobacteria 16s rRNA
Rec-I-DCM3 significantly improves performance Current best techniques DCM boosted version of best techniques Comparison of TNT to Rec-I-DCM3(TNT) on one large dataset
Rec-I-DCM3(TNT) vs. TNT(Comparison of scores at 24 hours) Base method is the default TNT technique, the current best method for MP. Rec-I-DCM3 significantly improves upon the unboosted TNT by returning trees which are at most 0.01% above optimal on most datasets.
Summary • Rec-I-DCM3 is a powerful technique for escaping local optima, and “boosts” the performance of the best heuristics for solving MP • The improvement increases with the difficulty of the dataset - Rec-I-DCM3(TNT) is 50 times faster than TNT on our hardest datasets, but we expect even bigger speedups in our next version • DCMs also boost the performance of Maximum Likelihood heuristics (not shown)
Acknowledgements • Collaborators: Bernard Moret (UNM), Usman Roshan (UT-Austin), and Tiffani Williams (UNM) • Funding: NSF, The David and Lucile Packard Foundation, The Radcliffe Institute for Advanced Study, The Institute for Cellular and Molecular Biology at UT-Austin, and The Program in Evolutionary Dynamics at Harvard University • Software will be part of the CIPRES Project’s first distribution - see http://www.phylo.org
Cyber-Infrastructure for Phylogenetic RESearch (http://www.phylo.org) • Main research: Large-scale phylogenetics, reticulate evolution, gene order phylogeny, and databases • Funded by $11.6M ITR Grant from NSF • 40 biologists, computer scientists, and mathematicians collaborating on the project