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High density microarray analysis of genes regulated by inflammatory stimuli.

High density microarray analysis of genes regulated by inflammatory stimuli.

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High density microarray analysis of genes regulated by inflammatory stimuli.

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  1. High density microarray analysis of genes regulated by inflammatory stimuli. Human peripheral blood monocytes (PBM) or human gingival fibroblasts (HuGi) were stimulated for the indicated times with LPS (100ng/ml), IL-1a (10ng/ml) or TNFa (20ng/ml) or were left untreated. Subsequently total RNA was isolated, converted into cRNA synthezised and labelled with Cy3 or Cy5, respectively. Labelled cRNAs of unstimulated and of stimulated cells were mixed and cohybridized onto MWG Biotech 30kA Arrays which carry oligonulelotide probes for 10.000 functionally characterized human genes. Depicted graphically are the fluorescence intensity values of all measured spots. Spots outside the red lines represent at least twofold differences between unstimulated and stimulated cells. Each measurement represents the mean of a replicate experiment, in which each sample was labelled once with Cy3 and once with Cy5 to exclude dye-artefacts. (Michael Kracht, Oliver Dittrich-Breiholz, unpublished data).

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