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Photosynthesis. Bio I Rupp. Focusing Questions. Where does the energy to do work come from? What chemical do cells use for energy? What is the equation for photosynthesis? How are chloroplasts arranged? What is NADPH? What are the two reaction pathways in photosynthesis?. Energy.
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Photosynthesis Bio I Rupp
Focusing Questions • Where does the energy to do work come from? • What chemical do cells use for energy? • What is the equation for photosynthesis? • How are chloroplasts arranged? • What is NADPH? • What are the two reaction pathways in photosynthesis?
Energy • Light or solar energy is converted to chemical energy • This occurs in the chloroplast of plants • Plants • Blue-green algae • Photosynthetic bacteria
History of Photosynthesis • Jan van Helmont • Belgian Physician • 1643 • Willow tree experiment • What caused the tree to gain weight?
History of Photosynthesis con’t. • Joseph Priestley • English minister and chemist • 1771 • Candle experiment • What did the plant produce?
History of Photosynthesis con’t. • Jan Ingenhousz • Dutch scientist • 1779 • Repeat of Priestley’s work • Plants only functioned in what?
History of Photosynthesis con’t. • Based on the experiments of van Helmont, Priestley, and Ingenhousz, what three things can we say plants need/produce?
Photosynthesis Equation • CO2 + H2O + Light C6H12O6 + O2
Leaf Structure • Epidermis—outer cells of leaf • Palisade mesophyll—where the majority of chloroplasts are located • Spongy mesophyll—some chloroplasts are located here
Leaf Structure con’t. • Xylem—water transport • Phloem—food transport • Cuticle—waxy covering that stops evaporation of water from the leaves • Stoma—opening in leaf that allows CO2 to enter
Chloroplast structure • Stroma—the fluid interior that surrounds the other chloroplast structures • Granum—stacks of disk shaped thylakoids • Thylakoids—the site of photosynthesis
Chloroplast structure con’t. • What makes the chloroplasts green? • Pigments • Chlorophyll a (650-700nm and 400-450nm) • Chlorophyll b (450-500nm and 650nm) • Carotenoids (450 nm) • Xanthophyll (600nm)
How does Photosynthesis Work? • Light is captured • Light energy is converted to chemical energy • The chemical energy is a combination of • ATP • NADPH • Sugars • Light reaction pathways • Dark reaction pathways
Light Reactions • Step one • Light hits photosystem II and boosts electrons to higher energy level • H+ ions are transported to create a concentration gradient • ATP is produced
Light Reactions con’t. • Step Two • Low energy electrons enter photosystem I and is re-energized • The re-energized electrons then move into the NADPH electron chain
Restoring Photosystem II • If electrons keep moving out where are the initial electrons coming from? • Water splitting reaction
H+ ions and ATP Creation • How did H+ ions move across the thylakoid membrane? • They were pumped by ATP synthase • Facilitated diffusion
Dark Reactions • Also known as the Calvin Cycle or Light Independent reactions • The ATP and NADPH from the light reactions is used to turn CO2 into sugar
Dark Reactions con’t. • Step one • Carbon fixation • CO2 is attached to a 5 carbon sugar called ribulose bisphosphate (RuBP) • The resultant molecule is unstable and splits into two 3-carbon molecules of PGA
Dark Reactions con’t. • Step two • Reduction • ATP and NADPH are used to rearrange the two 3-carbon molecules of PGA
Dark Reactions con’t. • Step three • Regeneration of RuBP • Two of the PGAL molecules are used to make glucose • The remaining PGAL is rearranged, with the help of ATP, to RuBP
Alternative pathways • C3 plants • Fix carbon to RuBP via the enzyme rubisco • Rubisco is probably the most abundant enzyme on Earth • If CO2 levels drop then the Calvin Cycle is starved • Photorespiration
Alternative Pathways con’t. • C4 plants • CO2 is fixed into a 4-carbon compound • The mesophyll cells store the CO2 to keep feeding the Calvin Cycle • Slows down photorespiration • Good method for plants in hot climates
Alternative Pathways con’t. • CAM plants • Crassulacean acid metabolism • CO2 is gathered at night and stored • CO2 can be released to keep the Calvin Cycle running • Photorespiration decreases
Factors Affecting Photosynthesis • Light intensity • CO2 levels • Temperature