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Actin-lysozyme complex in cystic fibrosis sputum

Actin-lysozyme complex in cystic fibrosis sputum. Sanders, L.K., et al., PNAS 104, 15994-9 (2007) “Control of electrostatic interactions between F-actin and genetically modified lysozyme in aquaous media” .

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Actin-lysozyme complex in cystic fibrosis sputum

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  1. Actin-lysozyme complex in cystic fibrosis sputum Sanders, L.K., et al., PNAS 104, 15994-9 (2007) “Control of electrostatic interactions between F-actin and genetically modified lysozyme in aquaous media” Charge-reduced lysozyme mutant (orange) and F-actin (blue) in end view. This structure is unstable. WT lysozyme (orange) and F-actin (blue) in end view. Stable structure, unaltered by mucin. This research confirms the existence of the lysozyme-actin complex in cystic fibrosis sputum and demonstrates that engineered lysozyme might prevent infected mucus molecules from forming ordered bundles that trap antimicrobials.

  2. Molecular organization of neuroligin/neurexin complex in synaps.Implication in neurodevelopment disorders, including autism spectrum disorder. Comoletti, D., et al., Structure 15, 693-705 (2007). Model of the neuroligin/b-neurexin assembly, deduced from solution scattering studies (left), and their possible configuration in the synaptic space (right).

  3. Structural Polymorphism of the Actin-Espin System: A Prototypical System of Filaments and Linkers in Stereocilia Espins are a type of cross-linking protein that are responsible for the formation of large parallel f-actin bundles. Scattering experiments have shown that normal espins cause the actin to arrange in highly ordered hexagonally packed rigid bundles, whereas a deafness causing mutation of the espin is able to induce only a weak nematic order, resulting in bundles of much lower stiffness. ( a ) hexagonally coordinated peaks are shown by packed F-actin-espin bundles ( b ); ( c ) the scatttering from the bundles with mutant espins only show nematic order ( d )‏ Kirstin R. Purdy, James R. Bartles, and Gerard C. L. Wong, Phys.Rev.Lett.98, 058105, 2007

  4. Synaptic Arrangement of the Neuroligin/b-Neurexin Complex Revealed by X-Ray and Neutron Scattering model of the neuroligin/neurexin complex in the synaptic cleft scattering data and shape reconstruction of the neuroligin/neurexin complex The tree dimensional solution structure of the neuroligin/neurexin complex, determined by SAXS and SANS methods, allowed for the development of a structural model of their spatial arrangement in the synaptic cleft. As mutations in the neroligin and neurexin gene are linked to autism, this provides a structural framework for understanding altered recognition by these proteins in neurodevelopmental disorders. Davide Comoletti, Alexander Grishaev, Andrew E. Whitten, Igor Tsigelny, Palmer Taylor and Jill Trewhella, Structure16, 693, (June 2007)‏

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