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Culture media ISO 11290

Culture media ISO 11290. PREENRICHMENT. Listeria Half Fraser Broth Base Cat. 1183. SELECTIVE ENRICHMENT. PLATE . CONFIRMATION. Culture Media in ISO 11290. Listeria Fraser Broth Base Cat. 1182. Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345. Motility.

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Culture media ISO 11290

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  1. Culture mediaISO 11290

  2. PREENRICHMENT Listeria Half Fraser Broth Base Cat. 1183 SELECTIVE ENRICHMENT PLATE CONFIRMATION Culture Media in ISO 11290 Listeria Fraser Broth Base Cat. 1182 Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 Motility Biochemical Test Hemolysis Carbohydrate utilization Listeria

  3. Listeria Fraser Broth Base Cat. 1182 Biochemical Test Allows the growth of Listeria with a black color, inhibiting the acompanying flora Culture Media in ISO 11290 PREENRICHMENT Listeria Half Fraser Broth Listeria Half Fraser Broth Base Cat. 1183 SELECTIVE ENRICHMENT Peptones / Salts / Buffer Lithium Chloride/ Nalidixic Acid Esculin/Ferric Amonium Citrate PLATE Incubate at 30ºC during 24 ± 2 hours Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 CONFIRMATION Esculin is hydrolized to esculetin, that reacts with ferric salts Nalidixic Acid inhibits Gram negative microorganisms Lithium Chloride inhibits Enterococcus that can hydrolize the Esculin

  4. Listeria Half Fraser Broth Base Cat. 1183 Listeria Fraser Broth Base Cat. 1182 Allows the growth of Listeria with a black color, inhibiting the acompanying flora Culture Media in ISO 11290 PREENRICHMENT Listeria Fraser Broth Base SELECTIVE ENRICHMENT Peptones / Salts / Buffer Lithium Chloride/ Nalidixic Acid Esculin/Ferric Amonium Citrate PLATE Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 Incubate at 35-37ºC during 24-48 hours CONFIRMATION Biochemical Test Esculin is hydrolizedto esculetin, that reacts with ferric salts Nalidixic Acid inhibits Gram negative microorganisms Lithium Chloride inhibits Enterococcus that can hydrolize the Esculin

  5. Listeria Fraser Broth Base Cat. 1182 Culture Media in ISO 11290 PREENRICHMENT Listeria Half Fraser Broth Base Cat. 1183 Listeria Agar Base Oxford SELECTIVE ENRICHMENT Peptones / Salts / Buffer Lithium Chloride/ Esculin/Ferric Amonium Citrate/Other antibiotics PLATE Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 Incubate at 32±2°C during 18-24 hours CONFIRMATION Biochemical Test Esculin is hydrolizedto esculetin, that reacts with ferric salts Cicloheximide inhibits yeast and molds Lithium Chloride inhibits Enterococcus that can hydrolize the Esculin Listeria monocytogenes and Listeria innocua grow giving the medium a black color

  6. Listeria Fraser Broth Base Cat. 1182 Esterase activity cleaves one of the cromogenic substrate, giving the colony a magenta color β-Galactosidase activity cleaves the other chromogenic substrate, giving the colony a green color Culture Media in ISO 11290 PREENRICHMENT Buffered Peptone water Cat. 1402 Listeria Chromogenic Agar Base SELECTIVE ENRICHMENT Peptones / Salts / Chromogenic Substrate Lithium Chloride/ Antibiotics PLATE Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 Incubate at 37ºC during after 24 ± 2 hours.Incubate the negative strains for 24±2 hours more CONFIRMATION Biochemical Test Salmonella growth in magenta colonies

  7. Hemolysis Listeria Fraser Broth Base Cat. 1182 Culture Media in ISO 11290 PREENRICHMENT Buffered Peptone water Cat. 1402 Motility SELECTIVE ENRICHMENT PLATE Listeria Agar Base Oxford Cat. 1133 & Listeria Chromogenic Agar Base Cat. 1345 CONFIRMATION Biochemical Test Carbohydrate Utilization

  8. Results

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