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University in Skopje Macedonia

University in Skopje Macedonia. Institute of Biology Faculty of Natural Sciences & Mathematics. Gill lesions in Prespa barbell (Barbus prespensis) inhabiting a polluted area of Lake Prespa. Danica Roganovic-Zafirova & Adriana Spasova. Scope and Objectives.

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University in Skopje Macedonia

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  1. University in Skopje Macedonia Institute of Biology Faculty of Natural Sciences & Mathematics

  2. Gill lesions in Prespa barbell (Barbus prespensis) inhabiting a polluted area of Lake Prespa Danica Roganovic-Zafirova & Adriana Spasova

  3. Scope and Objectives • Prespa barbel is near bottom dwelling fish • Gills are important respiratory and ion balance organs highly exposed to environment • Histopathological analysis of these organs was expected to provide some insight into level and character of pollution pressure experienced by this endemic Lake Prespa habitant

  4. Material and Methods Sampling site: Sirhan - Asamati Golema Reka entrance

  5. Material and Methods • Animals examined:Barbus prespensis - 35adult individuals, both males and females • Sampling time: spring - summer (1999-2000) • Light microscopy methods: standard H&E sections and plastic embedded semi-thin sections • Data analysis:histopathological quantificationaccording to Bernet et al. (1999); mucous and chloride cell stereometry; semi-quantitative scoring of infection level • Statistics:Pearson’s test

  6. Results The most prominent lesions observed

  7. Results • Circulatory disturbances: aneurysms

  8. Results • Regresive changes: respiratory epithelium necroses

  9. Results • Progressive changes: interlamellar epithelium proliferation and lamellae fusion

  10. Results • Frequentparasitic and fungal infections

  11. Results Histopathological quantification revealed…..

  12. Results 100.0 75.0 50.0 25.0 0.0 rIGC rIGR rIGP rIGI rYI

  13. Results Chloride and mucous cell counting

  14. Results Chloride cells: 3.31 (2.18/4.34)/ interlamellar space Moucous cells: 0.25(0.17/0.58) / interlamellar space

  15. Conclusions

  16. Prespa barbels collected from a polluted area of Lake Prespa exhibited various histopathological and cellular changes which may signify impaired respiration and ionic or acid-base balances.

  17. Histopathological quantification data and stereological analysis outcome suggest that effects of environmental toxicants could be the main reason of the observed condition.

  18. The presence of a fungal opportunistic infection in the fish respiratory system indicates that the analyzed fishes may suffer some kind of immunosupression.

  19. This study provided the first evidence of environmentally induced lesions in the Prespa barbel gills, which warns further work on health status estimation of this endangered endemic species.

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