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3730 自動定序儀 每週一清洗步驟流程

3730 自動定序儀 每週一清洗步驟流程. 依照上圖指示 先 Water wash 冷水共 3 次 後 Water wash 溫水共 3 次 總共 water wash 6 次 PS: 溫水水溫約 38℃ 使用無菌燒杯取 Milli-Q water 約 80ml 微波加熱後隔水降溫 , 取溫度計測量溫度後才可使用. Flush. 待以上步驟完成後 即可將舊的廢液盤更換 包括 :buffer.water.waste 廢液盤 PS: 記得 polymer.1X running buffer 皆需要先回溫 30 分鐘後才可更換喔 !.

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3730 自動定序儀 每週一清洗步驟流程

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  1. 3730自動定序儀 每週一清洗步驟流程

  2. 依照上圖指示先Water wash冷水共3次後Water wash溫水共3次總共water wash 6次PS:溫水水溫約38℃使用無菌燒杯取Milli-Q water約80ml微波加熱後隔水降溫,取溫度計測量溫度後才可使用

  3. Flush

  4. 待以上步驟完成後即可將舊的廢液盤更換包括:buffer.water.waste 廢液盤PS:記得polymer.1X running buffer皆需要先回溫30分鐘後才可更換喔!

  5. 每個月月初定期清洗polymer block syrring1.取無菌空針,抽取20ml Milli-Q water2.準備ㄧ個乾淨的空瓶裝廢液3.syrring fitting and waste fitting稍微轉開1/2~1/34.從Syrring fitting處,將water push 進去(PS:速度1秒1滴,不可過快)

  6. 說明完3730機本清洗步驟後簡單舉例來討論,當發生下列異常時正確處理流程之步驟為何及如何找出問題點?~~動腦時間到囉~~說明完3730機本清洗步驟後簡單舉例來討論,當發生下列異常時正確處理流程之步驟為何及如何找出問題點?~~動腦時間到囉~~

  7. 狀況ㄧ~ 此array view約後2/3處皆有黃色之backgroung

  8. 看過上圖後,正確之處理步驟為何? 需觀察哪些地方來協助找出問題點呢!

  9. 檢查及處理步驟~ 1.首先將觀察機台狀況 *3730及軟體是否有亮紅燈或Error顯示 *機台溫度.室內溫度.EPT current.Laser.CCD Tm.有無漏膠 *此份Array前後之毛細管狀況是否正常 2.到Run history處將此份樣品相關畫面用小畫家照相存檔下來 Ex:Array view/EPT view/Instrument view/Event and Log 3.確認樣品來源及客戶別 4.確認反應人員及沉澱上機人員操作有無問題 5.確認近期內有無更換反應/沉澱/上機之試劑 6.開啟分析軟體觀察定序報告是否有問題

  10. 7.區別好問題點為樣品本身或是機台-毛細管問題7.區別好問題點為樣品本身或是機台-毛細管問題 8.處理問題—重新run樣品測試或是Run HiDi確定毛細管狀態 9.並於上機本內以紅筆記錄異常狀況及處理流程 慢慢變厲害囉 但是還有ㄧ題喔… …….嘿嘿

  11. 案例二~3730機台及collection softward軟體亮紅燈 並顯示Error:Leak detected during capillary filling/Run failured

  12. 請大家動動腦,說出正確處理步驟囉…………………..88請大家動動腦,說出正確處理步驟囉…………………..88 別緊張,提示來囉…………..

  13. 樣品正在分析中的instrument view~ EP Voltage:8.0~8.5 KV EP Current:180~200 uA Laser Power:25~25.5 mW Laser Current:5.0~5.5 A Buffer Heater:30~35 ℃ Cell Heater:48.5~49 ℃ Oven Tm:60 ℃

  14. 正常之EPT vuew~ EPT Current Oven Tm Ovent Peiltier Tm Buffer Tm Cell Heater EPT Voltage CCD Tm Laser Power Ambient Tm Lser Current

  15. EPT current代表之意義~

  16. Event Log記錄~

  17. Polymer Upper Tubing Buffer Jar

  18. 想到答案了嗎? 你是哪一個呢………………加油喔

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