AJ10 Ubiquitin merge

1. AJ10 NeuNmerge SOD1 G85R (P-340) C A B E D F SOD1 G93A fast (P-136) G H I L J K SOD1 G93A slow (P-247) M O N P Q R AJ10 Ubiquitin merge U S T Supplementary Figure 2. AJ10 immunoreactivity is associated with degenerating MNs in ventral horn both in SOD1G85R,or SOD1G93Amice (fast and slow strains). Spinal cord grey matter is delimited by a dotted line. Lumbar spinal cord sections from end-stage (postnatal (P) days in parentheses) mice overexpressing either human SOD1G85R (A-F) or SOD1G93A (G-L and M-R for the fast and slow strains , respectively) were double immunostained with AJ10 (red) and the neuronal marker NeuN (green); the large amount of AJ10-immunopositive profiles seen in (A, D, G, J and M, P), do not co-localize with apparently healthy NeuN-posi-tive neuronal cell bodies (B, C, E, F, H, I, K, L, N, O, Q, R). By contrast, AJ10 immunopositive neuronal cell bodies (encircled in J-L and P-R) no not display NeuN immunoreactivity. In (S-U) AJ10 immunoreactivity (red) was colocalized with ubiquitin (green). Some of the AJ10-positive mutant SOD1 aggregates (encircled in S-U) exhibit intense ubiquitin immunoreactivity; however, AJ10- positivity associated with neuronal processes (arrows in S-U), do not show ubiquitin immunoreactivity. Scale bar in C and F = 100 µm; U = 10 µm.