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Diffusion Rate CHEMICAL DIFFUSION IN AGAR

Diffusion Rate CHEMICAL DIFFUSION IN AGAR.

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Diffusion Rate CHEMICAL DIFFUSION IN AGAR

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  1. Diffusion Rate • CHEMICAL DIFFUSION IN AGAR IF YOU CAN SEE THIS MESSAGE YOU ARE NOT IN “SLIDE SHOW” MODE. PERFOMING THE LAB IN THIS MODE WILL NOT ALLOW FOR THE ANIMATIONS AND INTERACTIVITY OF THE EXERCISE TO WORK PROPERLY. TO CHANGE TO “SLIDE SHOW” MODE YOU CAN CLICK ON “VIEW” AT THE TOP OF THE PAGE AND SELECT “SLIDE SHOW” FROM THE PULL DOWN MENU. YOU CAN ALSO JUST HIT THE “F5” KEY. Click Here To Start Experiment #1 Click here to print a Data Table you will need for this experiment

  2. Diffusion is a process of equalization which involves movement of molecules from an area of high concentration to an area of low concentration Click Mouse Here to View Next Text on the Blackboard

  3. This exercise investigates diffusion as it applies to movement of particles in a semi-solid material called agar Click Mouse Here to View Next Text on the Blackboard

  4. Molecules are in a constant state of motion The motion of these molecules are influenced by many factors Click Mouse Here to View Next Text on the Blackboard

  5. FACTORS INFLUENCING DIFFUSION 1) Kinetic Energy2) Nature of the environment3) Size of the molecules Click Mouse Here to View Next Text on the Blackboard

  6. KINETIC ENERGY The driving force which causes the molecules to move Click Mouse Here to View Next Text on the Blackboard

  7. NATURE OF THE ENVIRONMENT The agar environment we will be using is a semisolid material through which we will measure the rate of diffusion of substances with different molecular size Click Mouse Here to Perform the Exercise on Chemical Diffusion in Agar

  8. SIZE OF THE MOLECULES Smaller molecules move faster than larger molecules Click Mouse Here to View Next Text on the Blackboard

  9. LAB REPORTYou will need to supply the following in your lab report for this experiment #1 1) Data Table for Diffusion of Different Molecular Weights 2) Answers to questions 1-4 Click Mouse Here to Perform the Exercise on Chemical Diffusion in Agar Click here to print a Data Table you will need for this experiment

  10. Procedure • Obtain two test tubes with agar*You can obtain the test tubes by clicking on the test tube rack on the shelf to the left Test tube rack Click Mouse Here to View Next Instructions on the Blackboard

  11. Procedure 2) Mark the test tubes as#1 and #2*If you click on the pencil lying on the shelf to the left you can mark the tubes 1 2 Click Mouse Here to View Next Instructions on the Blackboard

  12. Procedure 3) Add a small amount of methylene blue to test tube #1*You can click on the blue eyedropper lying on the shelf to the left to add the dye 1 2 Click Mouse Here to View Next Instructions on the Blackboard

  13. Procedure 4) Quickly click on the START button on the timer and record on your printed Data Sheet every 15 minutes the distance the dye has moved through the agar*You can measure the progress of the dye by observing the mm marks on the test tubes Click here if you messed up and would like to try this experiment again 1 2 START 00:00 05:00 10:00 15:00 20:00 30:00 45:00 40:00 35:00 50:00 55:00 60:00 25:00 Click Mouse Here to View Next Instructions on the Blackboard

  14. Procedure 5) Add a small amount of potassium permanganate to test tube #2*You can click on the green eyedropper lying on the shelf to the left to add the dye 1 2 Click Mouse Here to View Next Instructions on the Blackboard

  15. Procedure 6) Quickly click on the START button on the timer placed on the table and record every 15 minutes the distance the dye has moved through the agar*You can measure the progress of the dye by observing the mm marks on the test tubes Click here if you messed up and would like to try this experiment again 1 2 START 00:00 05:00 10:00 15:00 20:00 30:00 45:00 40:00 35:00 50:00 55:00 60:00 25:00 Click Mouse Here to View Next Instructions on the Blackboard

  16. DIFFUSION OF DYE IN AGAR Click the Play button to view the diffusion of dye in an agar plate. Which chemical would you propose is smaller the RED or BLUE? Click Here to View the Next Slide

  17. DATA At this point you should have collected diffusion distance data for methylene blue and potassium permanganate Include your data tables with your lab report when you are finished Click Here to view a page to print for your Data Table Click Mouse Here to View Next Instructions on the Blackboard

  18. Questions Answer the questions (1-4) for this experiment Hand your answers in with your lab report • What are the three things which influence the movement of molecules (and particles)? • In agar, what size of molecules move the fastest? • Which dye, the methylene blue or the potassium permanganate, diffused at the fastest rate? • Why did one dye diffuse faster than the other? Click Mouse Here to View Next Instructions on the Blackboard

  19. At this point you have finished experiment #1 for this lab Would you like to end your session or continue to the next experiment? End this Session Continue to Experiment #2 (PASSIVE MOVEMENT) Continue to Experiment #3 (OSMOSIS AND DIALYSIS)

  20. You may also use this hyperlink to Print this Data Table if you are Online Click Here to Return to Experiment #1

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