1 / 7

Cytokines

Cytokines. Properties of Cytokines Cytokine Receptors Cytokine Antagonists Cytokine Secretion by TH1 and TH2 Subsets Cytokine-Related Diseases Therapeutic Uses of Cytokines and Their Receptors Cytokines in Hematopoiesis. 1.Properties of Cytokines.

charity-solis
Download Presentation

Cytokines

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Cytokines

  2. Properties of Cytokines Cytokine Receptors Cytokine Antagonists Cytokine Secretion by TH1 and TH2 Subsets Cytokine-Related Diseases Therapeutic Uses of Cytokines and Their Receptors Cytokines in Hematopoiesis

  3. 1.Properties of Cytokines • Overview of the induction and function of cytokines. • (b) Most cytokines exhibit autocrine and/or paracrine • action; fewer exhibit endocrine action.

  4. ELISA assay of a cytokine. (a) The sample containing the cytokine of interest is captured by specific antibody (blue) coated onto wells of a microtiter plate. A second specific antibody (blue), conjugated to an enzyme (E) such as horseradish peroxidase, forms a sandwich with the captured cytokine, immobilizing the enzyme in the microtiter well. A chromogenic substrate (S) is added, and the enzyme generates a color whose intensity is proportional to the amount of cytokine bound to the capture antibody. The optical density of this color produced by the unknown is compared with values on an appropriately determined standard curve. (b) The standard curve shown here is for human interleukin 12 (IL-12). It is clear that this assay is sufficiently sensitive to detect as little as 1 picogram of IL-12.

  5. 1) Cytokines Belong to Four Structural Families

  6. Cytokine attributes of (a) pleiotropy, redundancy, synergy (synergism), antagonism, and (b) cascade induction.

More Related