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ECEU692 Subsurface Imaging Course Notes Part 3: Imaging with Light (2) Hyperspectral Imaging. Profs. Brooks and DiMarzio Northeastern University Spring 2004. Hyperspectral Imaging Concepts. ER-2. Absorption Spectroscopy Scattering Reflectance Spectroscopy Imaging Hyperspectral Imaging
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ECEU692Subsurface ImagingCourse NotesPart 3: Imaging with Light (2)Hyperspectral Imaging Profs. Brooks and DiMarzio Northeastern University Spring 2004 Chuck DiMarzio, Northeastern University
Hyperspectral Imaging Concepts ER-2 • Absorption • Spectroscopy • Scattering • Reflectance Spectroscopy • Imaging • Hyperspectral Imaging • The Data Cube AVIRIS Chuck DiMarzio, Northeastern University
Beer’s Law Chuck DiMarzio, Northeastern University
Sample Absorption Spectra Data from Van Assendelft, O. W., Spectrophotometry of Haemoglobin Derivatives, Springfield, IL: Thomas, 1970. Graph by DiMarzio, Warner, Dwyer, Northeastern University, 1994 kaSpecific Absorption, /cm /M Hb HbO2 686 l, Wavelength, nm Chuck DiMarzio, Northeastern University
Light in a Turbid Medium • Specular Components • Fresnel Coefficients • Beer’s Law • Diffuse Components • More Complicated • Some Examples • Paint • Clouds Chuck DiMarzio, Northeastern University
Reflectance Spectroscopy;Weak Scattering Chuck DiMarzio, Northeastern University
A Simple Model ma(l1)=C1k1(l1)+ C2k2(l1) ma(l2)=C1k1(l2)+ C2k2(l2) Two Measurements, Two Equations, and Two Unknowns Later, Prof. Brooks will talk about inverting these equations Chuck DiMarzio, Northeastern University
No Hb HbO2 Hb Reflectance Spectroscopy;Strong Scattering Chuck DiMarzio, Northeastern University
Pulse Oximeter • 2 LED’s • Different IR Wavelengths • DC Components • Total Hb, HbO2, Other Absorbers • Pulsatile Component • Arterial Hb, HbO2 Chuck DiMarzio, Northeastern University
Hyperspectral Imaging Overview • Definition (Multi-, Hyper, Ultra-Spectral) • The Data Cube • Image Acquisition Hardware, Data Visualization • HOSIS - Hyperspectral Oxygen Saturation Imaging System: • CCD • Lyot Filter • Digital Imaging Chuck DiMarzio, Northeastern University
Hyperspectral Imaging • Definitions? • Imaging in multiple bands with width less than (10%?) of the center frequency • Imaging in more than (25?) bands • Imaging in Contiguous Bands y l x The Hyperspectral “Data Cube” Chuck DiMarzio, Northeastern University
y l x Hyperspectral Imaging Hardware Concepts (1) • Camera Coordinates are Spatial • Successive Images are Different Wavelengths B/W Camera Tunable Filter Chuck DiMarzio, Northeastern University
y l x Hyperspectral Imaging Hardware Concepts (2) • Pushbroom Scan • Airborne or Vehicle Mounted? • Camera Coordinates are y, l • Successive Scans are Different values of x Chuck DiMarzio, Northeastern University
HOSIS Instrumentation Block Diagram Processed Images Halogen Lamp CCD Camera Tunable Filter See Supplementary Notes Computer System with Video Board Target Tissue Thanks to Peter Dwyer at Lucid Technologies, MGH, and NU Chuck DiMarzio, Northeastern University
Quantitative Calculations Difficult Subject to Change Calibration Standards Light Level Reflectance Sources of Variation Light Source Camera Sensitivity Filter Losses Geometry Atmosphere? Other? Quantitative Imaging Chuck DiMarzio, Northeastern University