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PAL CHS CHR IFR COMT Fig S1 Gene expression analysis in split root systems. RNA was extracted from split root systems and transcript accumulation was analysed by real-time RT-PCR. Expression values were obtained from roots of split root systems where two halves were inoculated with F. (Fc and Fa) and after four weeks of mycorrhizal colonisation two halves infected with A. (Ac and Af). The other halves were mock-inoculated (C, Cf and Ca). Shown are mean values and standard deviations. All RNA accumulation values were normalised by the expression of the M. truncatula TEF1 gene. Significant interactions were detected for all genes. Different letters above the columns indicate significant differences between treatments (Fisher’s LSD; P = 0.05; n = 3).