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Would glucose or lactose be more beneficial for the growth of P. fluorescens ?. Hyun Suk Park Anh Trinh Carissa Best. Glucose. The Critter. Pseudomonas fluorescens Gram negative, rod-shaped Thrives in environments of 25 degrees Celsius
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Would glucose or lactose be more beneficial for the growth of P. fluorescens? Hyun Suk Park Anh Trinh Carissa Best Glucose
The Critter Pseudomonas fluorescens • Gram negative, rod-shaped • Thrives in environments of 25 degrees Celsius • Able to grow on a variety of media including lactose
Why we picked lactose? To examine the effects on growth rate in comparison to glucose. 20% concentration of Glucose OD Time(min)
Glucose vs. Lactose P. Fluorescens growth on lactose or media enriched culture OD -Glucose(3%) -Lactose(3%) Time(min)
Lactose concentrations Further explore an effective amount of lactose at the lowest concentration possible to steady the growth rate OD Time(min)
Conclusion • Lactose effectively controlled the overall growth of P. fluorescens in comparison to glucose within a certain range • At very low concentrations of lactose a more accurate transfer method is needed to have significant measures of the effects on overall growth
Next Time • Possible further experiments with additional time • Better dilution method/procedure • Lactose solution settling and preparation