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What Fish Is It?

What Fish Is It?. Using STEM to help ELL students advance. Presenters. Christine Mazza NYCDOE Office of School Programs and Partnerships MSP Grant Manager Painda Zaman John Adams H.S . Living Environment Teacher. MSP Grant. Federally Funded 3 year Grant Competitive Process

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What Fish Is It?

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  1. What Fish Is It? Using STEM to help ELL students advance

  2. Presenters Christine Mazza NYCDOE Office of School Programs and Partnerships MSP Grant Manager Painda Zaman John Adams H.S. Living Environment Teacher

  3. MSP Grant • Federally Funded 3 year Grant • Competitive Process • Minimum of 30 hours of Professional Development/year • Strives to: • Increase teacher content knowledge • Develop confidence teaching Science • Give teachers strategies to teach Science to a broad range of students

  4. MSP TRACKS • Focus on Wildlife • Urban Gardening • Composting • Wildlife Forensics • CSI-T • Shark Week! • Hands on! Minds on! • Operation Investigation

  5. Why STEM Education? STEM

  6. Understanding how to solve problems • By Using: • Science • Technology (Tools) • Engineering • Inventions • Designing • Mathematics • By speaking this new STEM language we can inspire the younger generation to develop new inventions to improve life

  7. symbols Throughout this presentation you will see 5 symbols. Those symbols represent the skillset required to complete the project: • Science • Technology • Engineering • Mathematics • Literacy

  8. Urban Barcoding A resource available for teachers in nyc

  9. The urban barcoding project • NYC Urban Barcoding Project • Students form research teams and decide which aspect of biodiversity in NYC they want to explore. • Teams submit a proposal for review. • Teams conduct field work • Collecting samples • Recording samples • Labeling and carefully storing samples

  10. The urban barcoding project • Teams begin lab work: • Extract DNA from samples collected • Amplify DNA using a PCR • Verify the amplified PCR using gel electrophoresis The DNA fingerprint

  11. The urban barcoding project • Teams send DNA samples to a lab for analysis • Results are electronically returned to teams • Teams trim and edit sequences on the DNA Subway • Teams align sequences • Teams analyze phylogenetic trees to determine species An example of a phylogenetic tree

  12. The urban barcoding project • Teams prepare and disseminate results • Teams present results to the UBP team • Oral presentation • Poster presentation • Final report of findings

  13. The urban barcoding project What is the cost for schools to participate in this project? ZERO! UBP provides: • Paid training for teachers • All lab equipment necessary for DNA extraction and analysis • Fees associated with species identification • Harlem DNA Lab

  14. What fish is it? Applying the urban barcode project to uncover a mystery

  15. Let’s fly to Bangladesh …and lets go fishing!

  16. Hilsa Fish • A tropical fish • National fish of Bangladesh • Large number of fish are caught in the Padma-Meghna-Jamuna delta, which flows into the Bay of Bengal • Rich in omega 3 fatty acids • Imported to the US to sell at local fish markets • Enjoyed locally here in the US by many Bengali residents

  17. What is the problem? Fish markets in NY import Hilsa fish from Bangladesh. The fish sold in NY does not taste like Hilsa fish sold in Bangladesh. Are the markets in NY swapping the fish out for a less expensive fish that looks similar to Bangladesh Hilsa fish?

  18. Who are the primary scientists investigating this mystery? Nabila Shabnam Safa Chowdhury Team Precious

  19. Their journey for solving the mystery • Team Precious and their teacher consulted with their mentor assigned to their proposal to discuss next steps. • The team gathered samples, labeled the samples and carefully stored them until they were ready to extract the DNA.

  20. Their journey for solving the mystery • Preparing the fish tissue sample. • Grinding the fish tissue sample with a pestle with nuclei lysis solution to begin to expose the DNA.

  21. Their journey for solving the mystery • After going through several more steps the sample is ready to be amplified in a PCR machine. • The team is now ready to analyze the PCR product using a gel electrophoresis to test DNA viability. • DNA is sent to a lab for analysis and results are returned electronically for the team to examine the phylogenetic trees to determine species.

  22. Sequencing the dna in the subway

  23. Sequence trimmer

  24. Pair builder- atcg

  25. Consensus editor

  26. blastn

  27. Phylogenetic tree

  28. The results • Utilizing several samples, it was determined that the Hilsa fish sold here in NYC, is in fact genetically linked to the Hilsa fish in Bangladesh. • The markets are not selling a cheaper fish.

  29. Possible causes for taste change • Fresh vs. frozen • Transit time • Area where the fish is caught – certain areas of the river produce better tasting Hilsa fish • Time of year when imported Hilsa is caught • Water from NYC used to clean and cook the fish • ….there are many variables that can alter the taste of this fish

  30. Meet the scientists http://vimeo.com/41430775

  31. Valuable resources • www.nyccsi.com • www.urbanbarcodeproject.org • http://dnasubway.iplantcollaborative.org • www.barcodeoflife.org • http://eol.org

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