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Zebra Fish

Zebra Fish. Danio rerio. What is a zebra fish?. Tropical freshwater fish Minnow family Generation time is ~3-4 months Adults ~ 4cm in length Omnivorous Eats primarily zooplankton, phytoplankton, insects and larvae. Why are zebra fish important?. The embryos are see-through

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Zebra Fish

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  1. Zebra Fish Danio rerio

  2. What is a zebra fish? • Tropical freshwater fish • Minnow family • Generation time is ~3-4 months • Adults ~ 4cm in length • Omnivorous • Eats primarily zooplankton, phytoplankton, insects and larvae

  3. Why are zebra fish important? • The embryos are see-through • allows us to watch the changes in development • Adults breed rapidly • good supply of embryos for our research • Embryos develop quickly • 4-5 days old the embryos become swimming larvae and start to catch their own food • Zebra Fish Development • Close homology to humans • More closely related to humans then models such as the worm and fruit fly, easier to use than mouse/rat

  4. UNFERTILIZED FERTILIZED Development HPF=Hours Post Fertilization 0 HPF 1 HPF 2 HPF 4 HPF 8 HPF 12 HPF 16 HPF 24 HPF 36 HPF 48 HPF 72 HPF

  5. Zebra in the classroom • Read through the zebra in the classroom article with a partner • What must be done in order for zebra fish to successfully breed? • Why are zebrafish embryos helpful for studying development? • What does hpf stand for? • What is it about zebra fish physiology that makes them good models to study in the lab? • Explain why biochemistry and genetics are important aspects to understand about the zebra fish. • Is it right to say that zebra fish are a “research tool”? • What information is most important to know for conducting an experiment with zebra fish?

  6. Salt Solution (Test Salinity) • Must use 3 Concentrations of your choice

  7. pH Solution (Test acidity) • Must Use 3 Concentrations of your choice • ADD ACID TO WATER NEVER WATER TO ACID!!!

  8. Ethanol Unstable…must make the day before use Must use 3 Concentrations of your choice

  9. Zebrafish Development - Zygote “The Bubble” • Zygote Period – 0 – 0.75 hpf • Fertilized Egg to develop with its first division of cells

  10. Cleavage Period “The bubbles boil over” • Cleavage - 0.75 – 2.25 hpf • Cells begin to divide… still large enough to count

  11. Blastula Period “The afro” • Blastula- 2.25-5.25 hpf • Cells multiply very rapidly at the top

  12. Gastrula Period – “The Solar Eclipse” • Gastrula - 5.25- 10.33 hpf • Outside of the cell begins to develop

  13. Segmentation Period “ The six pack abs” • Segmentation - 10.33 – 24.0 hpf • Somites form around the midsection for segmentation.

  14. Pharyngula Period “The fat belly” • Pharyngula - 24.0-48.0 hpf • Fish has large yolk sack present/ Body straightens

  15. Hatching Period “The free fish” • Hatching - 48.0-72.0 hpf

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