N. Kovatcheva, R.Vasilyev ,I. Zagorsky ., E.Haugan ., S . Ruud

1. LIVE STORED RED KING CRAB ( PARALITHODES CAMTSCHATICUS) – POSSIBILITIES AND CHALLENGES N. Kovatcheva,R.Vasilyev ,I. Zagorsky., E.Haugan., S. Ruud Russian Federal Research Institute of Fisheries and Oceanography(VNIRO), Moscow, RussiaNorway King Crab Production AS, Bugǿynes, NorwayTroika Seafood AS, Kirkenes, Norway

2. Laboratory of Crustacean Reproduction and Cultivation (VNIRO)

3. Achievements • Technology of red king crab artificial reproduction was developed and patented; • Several land and sea based complexes for live red king crab storage were planned and started on the Barents sea; • Red king crab heart activity monitoring system was tested and installed; • Technology for giant freshwater prawn cultivation was upgraded and patented;

5. Russian-Norwegian research project ( 2007-2011) : 1. «Conceptual engineering study (TDA) of land based red king crab live storage complex on the Barents sea» (2007). 2. «On-line optimization and biological experimentation on red king crab long term keeping in the land based storage complex (Bugǿynes, Norway)» (2008-2011). Norway King Crab Production AS, Bugǿynes, Norway VNIRO, Moscow, Russia

6. TASKS & GOALS: • Establishment of the experimental land based complex for red king crab keeping and ongrowing ; • Optimization of techniques for long term storage and ongrowing of thered king crab; • Development of effective methods for physiological control of crabs under keeping in the complex. • Upgradeof techniques for long term live king crab shipment.

7. Establishment of the experimental land based complex for red king crab keeping and ongrowing 1 Starting point. Not long ago…. Inside the complex

8. Complex structure (according to TDA made by VNIRO ) • Experimental tank room • for red king crab storage. • 2. Laboratory. • 3. Experimental aquarium • module. • 4. Utility rooms. • 5. Personal service room. 1 3 2

9. WHAT WE HAVE GOT TODAY • FLOW THROUGH WATER SUPPLY SYSTEM; • ON-LINE VITAL WATER PARAMETERS CONTROLING SYSTEM; • 39 TANKS SPECIALLY DESIGHNED FOR CRABS KEEPING; • 8 TANKS FOR INDIVIDUAL EXPERIMENTS IN AQUARIUM MODULE; • HEART ACTIVITY MONITORING SYSTEM INSTALLED; .

10. Optimization of techniques for long term storage and ongrowing of thered king crab 2 • Sorting, viability prognoses; • Densities; • Feeds and rations; • Meat content determination; • Storage during pre-moulting and moulting period. Research directions:

11. INCOME Fast discharge • size • shape • meat content Task: • Development of survivability prognosis technique Accurate sorting Quarantine Important to control catch process and delivery from the sea. Catch for production is not the same for keeping.

12. Permanent hydrological control Main hydrological parameters inside the complex (20.12.08-02.02.09)

13. Testing feeds and diets • Research items: • Preferences; • Gastrointestinal transit time; • Availability; • Feeding regimes ; Necessary to develop optimal receipt for artificial feeds for red king crab and other crustaceans

14. Meat content check studies 1. Palpation Very subjective and approximate method Invasive, dreadful, labor consuming, unprofitable 2. Boiling & instrumental measurement We need more accurate, effective and profitable method for measuring meat content

15. Measurement of meat content using photo fixation and computer processing of data • Main instruments: • digital camera; • Adobe Photoshop CS4; Lineal measurement scheme Fragment measurement scheme

16. Red king crab molting history (Inside complex, January - April 2009)

17. Molting indicators: • Aversion to food; • Shell soften; • Second shell; • Damages on legs; • Pumped abdomen. We need a technique for molting stage determination based on morphological and biochemical analysis

18. Development of effective methods for physiological control of crabs under keeping in the complex 3 Morphological and biochemical studies • Hemolymph • Hepatopancreas • Muscle tissue Hepatopancreas index of crabs (10/2008)

19. Monitoring of crabs heart activity under different stress factors: • light regime; • water flow speed; • dissolved oxygen concentration decrease; • feeding; • staying out of water; • handling. WELFARE

20. Dependence of the red king crab HRF on water temperature Changing in the crab HRF and SI under external impact in the process of cultivation in artificial conditions HRF and SI dynamics of red king crab in the feeding process Mechanical effect (cleaning of the tank) Dependence of the red king crab stress-index on water temperature

21. Upgradeof techniques for long term live king crab shipment. 4 Main goal – minimize crabs die off during long term transportation. • Main tasks: • Upgrading of packing methods; • Development of pre-delivery preparation methods; • Physiological control inside transportation box; • Morpho-physiological analysis before and after transportation.

22. Changing in the crab HRF and SI during simulative transportation

23. Mass media about the project

24. Norway King Crab Production AS may become an international red king scientific center. Bugǿynes is a very nice place for living and scientific work. Future is in international scientific collaboration in frame of business projects We are opened and what about you?

25. THANK YOU FOR YOUR ATTENTION! Tusen takk for oppmerksomheten!