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Life Science Lab BSC 1005L

Learn about the different objective lenses and magnification levels in a dissecting microscope, and how to use and care for the microscope.

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Life Science Lab BSC 1005L

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  1. Life Science LabBSC 1005L Lab 2 – The Microscope

  2. The Dissecting Microscope

  3. The Objective Lenses • Scanning Objective (4x) • Red color band, smallest one • Low-power objective (10x) • Yellow color band • High-power objective (40x) • Blue color band • Oil Immersion objective (100x) • Not used in this lab

  4. Magnification • Magnification = ocular x objective • Ocular lense is usually 10 • Examples: • Under 4x objective – magnification of specimen is 40 x • Under 10x objective – magnification of specimen is 100x • Under 40x objective – magnification of specimen is 400x

  5. Microscopy • Using the Microscope • Find the microscope # assigned to you in the cabinet • Carry the microscope with BOTH hands back to the lab bench • Plug it in, turn on light • Place slide on stage • Always view specimen on lowest power first! • Focus and center • Switch to higher power • Focus and center, etc. • When done: • Switch lens to lowest power!! • Take slide off • Clean lens with only lens paper • Turn light off • Unplug • wrap cord around base • Place back in cabinet

  6. Things to Remember • Be careful with Microscope • Never turn head around! • Always clean lenses with lens paper • In your drawer • You can clean slides with Kimwipes • Always put slides back in slide box in numerical order!!!!!

  7. Learn parts of the compound microscope that we will use • Learn how to make a wet mount • Refer to Figure 2.9 • Metric System • Be comfortable with measurements and know how to do conversions!

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