General Biology I Lab BSC 2010L

1. Lab 1 – Measurement and Microscopy General Biology I LabBSC 2010L

2. Welcome to Lab! • Be sure to familiarize yourself with safety procedures • Where does broken glass go? • What goes in the biohazard? • Etc. • Be sure to wear closed-toed shoes to lab everytime!

4. Properly reading Graduated Cylinder • Always use graduated cylinder to measure volume • Do not use a beaker!

5. Metric System • Conversions are in units of ten • Universal – used in all of the sciences

6. Metric System • Used in measurements of: • Length – Meter • Weight – Gram • Volume – Liter • Temperature – Celsius • °C = (°F – 32°)/1.8

7. Important Equations to Know • Area of a block = Length x width • Volume of a block = Length x width x height • 1cm3 (cc) = 1ml • Density = Mass/Volume • Displacement volume = • Total volume (after object) – Original volume (before object) • Temperature conversions: • °C = (°F – 32°)/1.8

8. Practice Problems • 51 cm = ______mm • 92 cm and 1 mm = ______mm • 72 mm = _____cm • 317 m = _____cm • 1.35 km = _____m • 4156 cm = _____m • 8000 m = ______km • 83 g = _____mg • 976 g = _____mg • 4000 mg = _____g • 3000 g = _____kg • 8 L = _____ml • 1.75 L = _____ml • 985 ml = _____L • 7428 ml = _____L

9. Practice Problem Answers • 510 mm • 921 mm • 7.2 cm • 31700 cm • 1350 m • 41.56 m • 8 km • 83000 mg • 976,000 mg • 4 g • 3 kg • 8000 ml • 1750 ml • 0.985 L • 7.428 L

10. Microscopy • Light Microscope • Binocular dissecting microscope • Compound light microscope • Electron Microscope • Transmission Electron Microscope • Thin slices of specimen • Scanning Electron Microscope • Image of surface of specimen

12. The Objective Lenses • Scanning Objective (4x) • Red color band, smallest one • Low-power objective (10x) • Yellow color band • High-power objective (40x) • Blue color band • Oil Immersion objective (100x) • Gray color band • Only use with oil! Have to carefully clean oil off afterwards!!!!!

13. Magnification • Magnification = ocular x objective • Ocular lense is usually 10 • Examples: • Under 4x objective – magnification of specimen is 40 x • Under 10x objective – magnification of specimen is 100x • Under 40x objective – magnification of specimen is 400x

14. Microscopy • Using the Microscope • Find the microscope # assigned to you in the cabinet • Carry the microscope with BOTH hands back to the lab bench • Plug it in, turn on light • Place slide on stage • Always view specimen on lowest power first! • Focus and center • Switch to higher power • Focus and center, etc. • When done: • Switch lens to lowest power!! • Take slide off • Clean lens with only lens paper • Turn light off • Unplug • wrap cord around base • Place back in cabinet

15. Things to Remember • Be careful with Microscope • Never turn head around! • Always clean lenses with lense paper • In your drawer • You can clean slides with Kimwipes • Always put slides back in slide box in numerical order!!!!!

17. Learn parts of the compound microscope that we will use • Learn how to make a wet mount • Refer to Figure 2.9 • Metric System • Be comfortable with measurements and know how to do conversions!