1 / 15

Visualizing Angiogenesis

Visualizing Angiogenesis. A CREST Initiative. Elise A. Pellmann – Marquette University Daniel Sem, Ph.D. – Concordia University Wisconsin David Goodsell, Ph.D. – Scripps Research Institute. 1. Cellular landscape. 2. Movie. 1. Cellular landscape. 2 x 10 7 magnification Show:

cicily
Download Presentation

Visualizing Angiogenesis

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Visualizing Angiogenesis • A CREST Initiative Elise A. Pellmann – Marquette University Daniel Sem, Ph.D. – Concordia University Wisconsin David Goodsell, Ph.D. – Scripps Research Institute

  2. 1. Cellular landscape 2. Movie

  3. 1. Cellular landscape • 2 x 107 magnification • Show: • MAPK and receptor tyrosine kinase signaling • VEGF, plurality of biochemical cascades • angiogenesis • cell permeability

  4. Knowledge needed for landscape • pathway: which proteins? • size, domain arrangement/shape, assembly, modifications, interactions/function

  5. Pathway: KEGG

  6. Pathway

  7. Proteins (i)

  8. Proteins (ii)

  9. Sketches

  10. Final version

  11. PyMOL, 3D Show: drug binding to DUSP-5 2. Movie

  12. ERK-2 phosphatase: P-T183, P-Y185 C-terminal phosphatase domain N-terminal ERK-binding domain linker region Ser to Pro mutation (hemangiomas) DUSP-5

  13. C: PDB 2G6Z • N: Homology model, NMR • Linker: hand-drawn • Domain orientation: good guess? N DUSP-5 model + + ERK-binding domain – – C catalytic domain

  14. ERK binding • catalytic domain “clamps” down • phosphates removed from ERK N DUSP-5 model Drug impedes catalytic action, may also prevent ERK binding if DUSP-5 clamps down on drug + + ERK-binding domain – – C catalytic domain

  15. Daniel Sem, Terrence Neumann, Prachi Pradeep David Goodsell Margaret Franzen, MSOE CREST grant Thank you Movie viewing

More Related