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Project Number: 2.5.10 and 2.5.12

Project Number: 2.5.10 and 2.5.12. Identify organs susceptible to encapsulated toxicants and technologies using oral delivery platforms. Jon J. Amberg , PhD, James A. Luoma , Terrance D. Hubert, PhD and Mark P. Gaikowski Upper Midwest Environmental Sciences Center, La Crosse, WI.

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Project Number: 2.5.10 and 2.5.12

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  1. Project Number: 2.5.10 and 2.5.12 Identify organs susceptible to encapsulated toxicants and technologies using oral delivery platforms Jon J. Amberg, PhD, James A. Luoma, Terrance D. Hubert, PhD and Mark P. Gaikowski Upper Midwest Environmental Sciences Center, La Crosse, WI

  2. Introduction and Objectives • Project Goal: Develop a management tool that minimizes impacts on native species • Toxicity: Depends on A-D-M-E • Objective: Identify weak points in biology/physiology • Planktivory and stomachless • Gill morphology, digestive enzymes, enteric microbiota • Pharmacokinetics • Detoxification enzymes

  3. Introduction and Objectives • Significance: • Expedite identifying new piscicides • Improve selectiveness for Asian carp • Partners: • Illinois Natural History Survey • Purdue University • University of Wisconsin at La Crosse • University of Illinois at Urbana-Champaign

  4. Current Knowledge • Digestive Physiology • Graduate student (gill morphology) • Sampled August and September 2011 • Methods developed • 19 digestive enzymes • Compared SC, GS and BMB • Some Findings: Elevated phosphatases, trypsin and lacking β-glucoronidase

  5. Current Knowledge (continued) • Pharmacokinetics • Completed preliminary study • Rotenone (silvers -vs- bigheads) • Developed qPCR assays • 13 genes (ETC, detox., O2 stress) • Developed methodology to quantify rotenone in plasma • Performed full exposure study • Rotenone (silver carp -vs- bigmouth buffalo) • Determined target concentration of rotenone in plasma

  6. Future Plans SC • Digestive Physiology • Monthly sampling • Dr. Greg Sass (Illinois Natural History Survey) • Enteric bacteria community structure • Dr. Wen-Tso Liu (University of Illinois at Urbana-Champaign) • Expand sampling into Wabash River • Dr. Reuben Goforth (Purdue University) BMB GIZ forefut Add timeline calendar

  7. Future Plans (continued) • Pharmakinetics • Testing additional chemicals • Antimycin-A, GD-174, ??? • Develop a more complete detox assay (increase # of genes analyzed) • Obtain sequence information for natives

  8. Technological or Other Hurdles • Sequencing (bigmouth buffalo and gizzard shad) • Methods to quantify other potential piscicides • Manpower 2 New Hires: Nate Jensen (2/2011) Kerry Weber (3/2011)

  9. Data Gaps • Reach differences • Upstream -v- downstream • Differences among life stages • Embryo, larvae, juvenile, adult • Other fishes? • Planktivorous larvae • Invertebrates?

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