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Supp. Table I

Supp. Table I. Dearth et al. 5. Supp. Table II. Dearth et al. 6. Supp. Fig. 1. Dearth et al. 7. A. wt p53. p53 DBS. p53 mutant. p53 DBS. p53. ADH1. URA3. p53mut. ADH1. CEN. LEU2. CEN. HIS3. CEN. TRP1. URA3. CEN. TRP1. MAT a. MAT a. MAT a. p53. ADH1. p53mut.

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Supp. Table I

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  1. Supp. Table I Dearth et al. 5

  2. Supp. Table II Dearth et al. 6

  3. Supp. Fig. 1 Dearth et al. 7 A wt p53 p53 DBS p53 mutant p53 DBS p53 ADH1 URA3 p53mut ADH1 CEN LEU2 CEN HIS3 CEN TRP1 URA3 CEN TRP1 MATa MATa MATa p53 ADH1 p53mut ADH1 CEN LEU2 CEN HIS3 URA3 p53mut ADH1 URA3 CEN TRP1 CEN HIS3 CEN TRP1 diploid diploid

  4. Supp. Fig. 1 Dearth et al. 8 B Type IV Collagenase Fas (APO-1/CD95) KILLER/DR5 p21, 5’ site p21, 3’ site GADD45 cyclin G MDM2 mdr1b GML CSR wt (eng) R248Q R273C R282W Y220C V157F E285K Y234C Y205C R158L C141Y P151S V272M P278L P278S H193R wt (nat) C wt (eng) R248Q R273C R282W Y220C V157F E285K Y234C Y205C R158L C141Y P151S V272M P278L P278S H193R wt (nat)

  5. Supp. Fig. 1 Dearth et al. 9 D wild-type R282W R248Q R273C E285K Y205C Y220C Y234C V157F wild-type H193R P278S P151S R158L P278L C141Y V272M

  6. Supp. Fig. 2 Dearth et al. 10 a topo II a topo II a tubulin a tubulin a p53 a p53 wt p53 N C control N C R175H N C R248Q N C R248W N C R282W N C R249S N C wt p53 N C C176F N C M237I N C E285K N C H179R N C G245D N C H179Y N C wt p53 N C Y234C N C R248L N C R280T N C P151S N C C238Y N C P278L N C wt p53 N C C135Y N C P151H N C V143A N C C257Q N C P151R N C

  7. Supp. Fig. 3 Dearth et al. 11 A Relative Activity 1.5 2.0 2.5 0 0.5 1.0 empty p21, 5’ site 37˚C wild-type R282W E285K Y234C V272M R283H M237R B 1.5 2.0 2.5 0 0.5 1.0 empty Noxa 37˚C wild-type R282W E285K Y234C V272M R283H M237R

  8. Supp. Fig. 4 Dearth et al. 12 A Relative Activity 1.5 2.0 0 0.5 1.0 p21, 5’ site 30˚C empty wild-type R282W E285K Y234C V272M B Relative Activity 1.5 2.0 0 0.5 1.0 Noxa 30˚C empty wild-type R282W E285K Y234C V272M C Relative Activity 0.6 1.0 1.4 0 0.2 0.4 0.8 1.2 1.6 30˚C wild-type cyclin G R282W wild-type GADD45 R282W wild-type GML R282W wild-type KILLER/DR5 R282W wild-type MDM2 R282W wild-type Pidd R282W

  9. Supp. Fig. 5 Dearth et al. 13 Class 1 R248Q Relative Activity wild-type anti-p53 mutant 0.8 1 1.2 1.4 0 0.2 0.4 0.6 10 0 10 10 10 50 Noxa 10 100 10 250 0 250 10 0 10 10 10 50 PCNA 10 100 10 250 0 250

  10. Supp. Fig. 6 Dearth et al. 14 Class 2 R273C R158L Relative Activity Relative Activity wild-type anti-p53 anti-p53 mutant 0.8 1 1.2 1.4 0 0.2 0.4 0.6 0 0.2 0.4 0.6 0.8 1 1.2 1.4 10 0 10 10 10 50 Noxa 10 100 10 250 0 250 10 0 10 10 10 50 PCNA 10 100 10 250 0 250 Class 3 R282W Y234C Relative Activity Relative Activity wild-type anti-p53 anti-p53 mutant 0.8 1 1.2 1.4 0 0.2 0.4 0.6 0 0.2 0.4 0.6 0.8 1 1.2 1.4 10 0 10 10 10 50 Noxa 10 100 10 250 0 250 10 0 10 10 10 50 PCNA 10 100 10 250 0 250

  11. Supp. Fig. 7 Dearth et al. 15 Class 5 Y220C Y163C Relative Activity Relative Activity wild-type anti-p53 anti-p53 mutant 0.2 0.4 0.6 0.8 1 1.2 1.4 0 0 0.2 0.4 0.6 0.8 1 1.2 1.4 10 0 10 10 10 50 GML 10 100 10 250 0 250 10 0 10 10 10 50 MDM2 10 100 10 250 0 250

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