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Electrochemistry upfront MS

Electrochemistry upfront MS. ROXY ™ - EC/LC upfront MS. With ReactorCell ™ for Generating Redox metabolites Cleavage of proteins/peptides Signal enhancement in MS Oxidative stress Metabolism. Why Electrochemistry / MS?. Mimics Natures Redox Reactions In Drug Development and Proteomics

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Electrochemistry upfront MS

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  1. Electrochemistry upfront MS

  2. ROXY™ - EC/LC upfront MS With ReactorCell™ for Generating Redox metabolites Cleavage of proteins/peptides Signal enhancement in MS Oxidative stress Metabolism

  3. Why Electrochemistry / MS? • Mimics Natures Redox Reactions In Drug Development and Proteomics • Fast & Clean • Complementary to existing methods

  4. Why Electrochemistry / MS? • Mimics Natures Redox Reactions • Drug development • Immediate results, minutes vs weeks • No by products, no cleanup • No biohazard • No rodents • Proteomics • No enzymes, clean without by products

  5. Electrochemistry / MS Mimicking nature’s redox reactions Fast alternative for laborious, time consuming procedures

  6. Electrochemistry / MS EC MS Mimicking nature’s redox reactions Fast alternative for laborious, time consuming procedures

  7. Modes of operation Off-line EC/MS On-line EC/MS • On-line EC/LC/MS • Pre injection • - Post column

  8. Electrochemical Reaction ΔE

  9. 100 80 60 Signal (%) 40 20 0 0 400 800 1200 1600 E (mV) Role of electrode potential E • Facilitates the EC reaction formation of oxidation or reduction products no reaction A  B

  10. 100 80 60 Signal (%) 40 20 0 2000 0 400 800 1200 1600 E (mV) Role of electrode potential E • Facilitates the EC reaction formation of secondary products B C A  B  C B: instable intermediate

  11. How to Find Optimum ΔE? Traditional Electrochemistry: Voltammogram On-line EC/ESI-MS: “MS Voltammogram” oxidative degradation of the substrate oxidationproducts 5000 oxidation products 4000 3000 E [mV] intensity [-] 2500 2000 2000 1000 1500 0 180 200 220 240 260 280 300 320 360 380 400 340 m/z

  12. OH R-CH2-CH3 R-CH-CH3 N-CH2-R NH + R-CHO R-O-CH2-R R-OH + RCHO O HO OH C=C C C C C R R R O- H R C NH R R C+N R R R Adapted from W. Lohmann et al. LC/GC Jan. 2010

  13. Proteomics • Electrochemical cleavage (oxidation) of proteins/peptides • ‘enzymatic digestion’ by electro-chemical cell • no enzymes, no non-specific cleavage, no auto-digestion, etc. 13

  14. Electrochemistry in Proteomics

  15. Electrochemistry in Proteomics

  16. Electrochemistry in Proteomics

  17. Conclusion EC/MS Many application areas Time and cost savings... Fast and clean

  18. Conclusion EC/MS • Mimics Natures Redox Reactions • Drug development • Immediate results, minutes vs weeks • No by products, no cleanup • No biohazard • No rodents • Proteomics • No enzymes, clean without by products

  19. www.myAntec.com Thank you!

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