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Time of Death: . During an autopsy, the forensic examiner wants to determine when the person died. . Time of death can: Prove innocence based on time and place or add interest. Many factors are used to approximate the time of death. Livor Mortis: Lividity (Purple color)
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Time of Death: During an autopsy, the forensic examiner wants to determine when the person died. Time of death can: Prove innocence based on time and place or add interest
Many factors are used to approximate the time of death • Livor Mortis: Lividity (Purple color) • Rigor Mortis (stiffness) • Algor Mortis (loss of temp) • Environment: ambient temp,humidity, wind • Clothing • Exercise • Weight • Decomposition of Eye?
Livor Mortis means death color As a body decomposes, blood seeps down through the tissues and settles into the lower parts of the body. RBC begin to break = spilling contents Hemoglobin turns purple when outside of RBC Purple color is visible on skin where blood pools
Lividity:the pooling of blood provides a clue as to how long the person has been dead First begins 2 hrs after death Discoloration becomes permanent after 8 hours If death occurred between 2 and 8 hrs lividity will be present but if the skin is pressed the color will disappear After 8 hours, lividity remains Ambient Temperature at death impacts the time it takes for lividity to set in Faster = hot Slower = cold **** important to note environmental condition surrounding body
Livor mortis provides clues in addition to time of death Position of corpse in the first 8 hours Reveal if the body has been moved Dual lividity: Ex. Person died sitting in a chair: lividity on back of thighs, buttocks and bottom of feet (in first 8 hours) Then the corpse is moved face down on the floor: lividity on face, chest and abdomen and front surface of legs Tight watches or belts affect livor mortis = impede blood flow
Rigor Mortis:temporary death stiffness • Starts within 2 hours • Starts in the head working down to the legs • After 12 hours, most rigid state • After 24-36 hours gradually lose stiffness due to acid formation (degradation) • Rigidity in face and neck: recent (2 hrs) • Body is rigid but not in face then over 15 hrs • If a body shows no rigor death occurred less than 2 hours or more than 48 Rigor also depending on the condition of the body at death and on factors in the atmosphere, particularly temperature.
Molecular chemistry - STIFF • Stiffness = • level of ATP dropping. • 2. myosin binds to actin and the muscles contract. • 3. However, with no ATP to reset the crossbridges and release the myosin • 4. Due to Ca2+ accummulation
Muscles eventually relax when apoptosis occurs (autolysis) Cell dissolves
Many factors affect When rigor mortis sets in and how long it lasts • Ambient temperature: cooler = slower • Weight: body fat stores extra O2 slows down rigor • Type of clothing: s keep body warm = accelerates rigor • Illness: fever: accelerates rigor, hypothermia: slower • Level of physical activity shortly before death: exercise, struggling speeds up rigor a. body temp b. less O2 c. increase in lactic acid • Sun exposure: increase rigor
Algor mortis: “death heat” • Temperature loss in a corpse • Insert a thermometer into the corpse’s liver • ~ 1 hr after death • Body cools at a rate of 0.78 oC (1.4oF) per hour • ~ After the first 12 hours • Body cools at a rate of 0.39 oC (0.7oF) per hour • Continues until reaches ambient temp of enviro. • ~ Environment affects rate of temp loss as well • Temp, Wind, excess body fat, clothing Rule of thumb estimate: lose 1 degree/hour for a range
Stomach and Intestinal Contents • 4 – 6 hours for the stomach to empty contents into S.I. • Another 12 hours for food to leave the S.I. • ~24 hours for a meal to be eaten and undigested food leaves the L.I. • Undigested contents in stomach: death <2 hours after last meal • Stomach is empty , food in S.I: death at least 4 – 6 hours after meal • Empty SI but wastes are in L.I. then death occurred > 12 hours
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