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Fluorescence Filters and Configurations for LSRII and FACSAria Flow Cytometers

This guide provides information about the fluorescence filters, configurations, and dyes used in LSRII and FACSAria flow cytometers. It includes details on antibody conjugates, DNA probes, functional dyes, and calcium flux assays.

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Fluorescence Filters and Configurations for LSRII and FACSAria Flow Cytometers

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  1. LSRII default 488 BLUE 488nm Default LSR II Configuration PerCP, PerCP-Cy5.5, PE-Cy5 FITC Alexa488 695/40 685 LP 530/30 505LP 488/10 550 LP SSC 735 LP 576/26 780/60 PE PE-Cy7

  2. LSRII default 633, 407 Default LSR II Configuration For A700 we need: 710/50, LP685 in B RED 633nm APC VIOLET 407nm PacificBlue, Alexa405 660/20 440/40 735 LP 505 LP 780/60 525/50 AmCyan, Alexa430, Cascade Yellow, CFP APC-Cy7, A750

  3. LSRII default 355 Default LSR II Configuration Hoechst (blue) DAPI UV 355nm 440/40 505 LP 530/30

  4. LSRII YFP/GFP GFP/YFP LSR II Configuration PerCP, PerCP-Cy5.5, PE-Cy5 GFP 695/40 685 LP 510/20 505LP 488/10 SSC 525 LP 735 LP 550/30 780/60 YFP PE-Cy7

  5. LSRII calcium, SP Stem Cell Side Population Dead Cell Exclusion LSR II Configuration for Calcium Flux Assays Hoechst (blue) DAPI Indo-1 violet UV 355nm UV 355nm 440/40 405/20 505 LP 450 LP 585/42 530/30 Hoechst (red) PI (UV) Indo-1 blue

  6. Aria default 488 BLUE 488nm Default FACSAria Configuration Use Aria 7-3-08 PerCP, PerCP-Cy5.5, PE-Cy5 PE 695/40 655 LP 576/26 556LP 530/30 502LP FITC Alexa488 488/10 SSC 595 LP 755 LP 610/20 780/60 PE-TexasRed PE-Cy7

  7. Aria default 633, 407 Default FACSAria Configuration PacificBlue, Alexa405 Hoechst Blue, DAPI Alexa700 APC-Cy5.5 VIOLET 407nm RED 633nm 720/20 440/40 680 LP 735 LP 502 LP 660/20 780/60 585/42 AmCyan, Alexa430, Cascade Yellow, CFP, Pacific Orange APC Alexa647 APC-Cy7

  8. Aria GFP/YFP FACSAria GFP/YFP Configuration PerCP, PerCP-Cy5.5, PE-Cy5 YFP 695/40 685 LP 550/30 525 LP 510/20 505LP GFP 600 LP 735 LP 610/20 780/60 PE-TexasRed PE-Cy7

  9. Aria SP FACSAria Stem Cell Side Population Dead Cell Exclusion VIOLET Hoechst Blue 440/40 505 LP 585/42 Hoechst Red PI (violet)

  10. Dyes, Alternative Names, Filters Part I Antibody Conjugates • FITC 530/30 • PE 576/26 • PE-TexasRed (ECD, Red610) 610/20 • PerCP 695/40 • PerCP-Cy5.5 695/40 • PE-Cy5 (CyChome, PC5, TriColor, Red670) 695/40 • PE-Cy7 780/60 • APC 660/20 • APC-Cy5.5 720/20 • Alexa700 720/20 • APC-Cy7 (PharRed) 780/60 • AmCyan 530/30 • Alexa430 530/30 • PacificBlue 440/40 • Alexa405 440/40

  11. Dyes, Alternative Names, Filters Part II DNA Probes, Fluorescence Reporters, Functional Dyes • GFP with YFP: 510/20 (change mirror to 525LP) only GFP: 530/30 • YFP with GFP: 550/30 (change mirror to 525 LP) only YFP: 576/26 • DsRed2 Aria: 610/20 or 576/26 LSR II: 576/26 or 585/42 (change PE filter) • CFP 530/30 (violet laser) • Hoechst 33342 blue 440/40 • Hoechst 33343 red 585/42 • PI (blue laser) Aria: 610/20 (PE-TexasRed) LSR II 695/40 (PerCP) CellCycle • PI (Aria violet or LSR II UV) 585/42 Dead Cell Exclusion • Indo-1 Calcium dye blue LSR II only 405/20 (insert 450 LP mirror instead of 505 LP!) • Indo-1 Calcium dye violet LSR II only 530/30 (insert 450 LP mirror instead of 505 LP!)

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