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INTERREG IVA project CRESH Cephalopod Recruitment from English Channel Spawning Habitats

INTERREG IVA project CRESH Cephalopod Recruitment from English Channel Spawning Habitats Céphalopodes: Recrutement Et Suivi des Habitats des pré-recrues de Manche. Task 7 progress : Tracking juvenile origin with trace elements. Task 7: Tracking juvenile origin with trace elements.

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INTERREG IVA project CRESH Cephalopod Recruitment from English Channel Spawning Habitats

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  1. INTERREG IVA project CRESH Cephalopod Recruitment from English Channel Spawning Habitats Céphalopodes: Recrutement Et Suivi des Habitats des pré-recrues de Manche Task 7 progress : Tracking juvenile origin with trace elements

  2. Task 7: Tracking juvenile origin with trace elements • Introduction : • Task 7 programme says that we shall be: • "Tracking juvenile origin with trace elements and stable isotope analysis of hard structures (among beaks, gladius, cuttlebone, statoliths)" • stable isotope data will come from task 5 • hard structures are too light for trave elements composition (given the available analysis systems)

  3. Task 7: Tracking juvenile origin with trace elements Introduction : At the University of Caen, scientists specialized in trace elements analysis are in the team "ERPCB" (Equipe de Recherche en Physico-Chimie et Biotechnologies) 2 meetings were organised with this team (May 5th 2010 and July 12th 2010) The contact person for this study is now Mrs. Christelle Caplat

  4. Task 7: Tracking juvenile origin with trace elements What are our objectives : To compare the composition of 4 different samples (4 samples of juveniles caught inshore + 1 recruits) Just a with genetic markers, better discrimination can be achived with a higher number of variables.

  5. Task 7: Tracking juvenile origin with trace elements • What trace elements are worth analysing ? • + A literature review suggests that the elements that are lost likely bioaccumulating in Cephalopods are • Cd, Hg, Ag, Zn, Cu (in decreasing order) • Problems with bioaccumulations: • assumes that contamination varies • detoxication processes may occur (not well known in Cephalopods … • (S. Seixas and P. Bustamante contacted about this)

  6. Task 7: Tracking juvenile origin with trace elements What methdology ? + The different methods (Mass-Spectrometry) have been discussed → ICP-OES (ICP "optique") qui est moins sensible que l'ICP-MS (dont on ne dispose pas) → SAA-ET(Spectrométrie d’Absorption Atomique avec atomisation électrothermique à l’aide d’un four graphite) qui est plus sensible que l'ICP-OES et multi-élementaire (alors que la SAA-flamme est mono-élementaire par rapport à l'ICP-OES) The main idea is to start with a method that enables to scan a series of elements (ICP-OES) which may reveal differences in other elements than the initial list.

  7. Task 7: Tracking juvenile origin with trace elements What methodology ? + the quantity of material needed for 1 analysis is (1< x < 2g dry matter) this will require pooling specimens + the validation of measures requires certified controls (same taxa ? or same trophic level?) + Literature review under way about differences in contamination levels (within the Channel)

  8. Task 7: Tracking juvenile origin with trace elements What are the next steps ? + collection of juvenile specimen (crucial !) (september - october) + collection of recruits sample (december -january) + processing of the samples

  9. Thank you for your attention…

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