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Albumin MIP Prepared in Aqueous Solution System

Albumin MIP Prepared in Aqueous Solution System. Speaker : Thong-Yueh Lin 報告者 : 林 聰 樂. Outline. Introduction Experimental Results and discussion Conclusions. Introduction. Property of Albumin. Half-life: 17 to 23 day Molecular weight: 68,000 Isoelectric point: 4.8

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Albumin MIP Prepared in Aqueous Solution System

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  1. Albumin MIP Prepared in Aqueous Solution System Speaker : Thong-Yueh Lin 報告者 : 林 聰 樂

  2. Outline • Introduction • Experimental • Results and discussion • Conclusions

  3. Introduction

  4. Property of Albumin • Half-life: 17 to 23 day • Molecular weight: 68,000 • Isoelectric point: 4.8 • Concentration of human serum: 3.0 to 5.0 g/dl

  5. Experimental

  6. Materials Monomer 3-dimethylaminopropyl metacrlamide:DMAPMA acrylamide:AM Allylamine:AA Methacrylic acid:MAA Hydroxy ethyl methacrylate:HEMA

  7. Materials Crosslinking agent Tetraethylene glycol dimethacrylate:TEGDMA Polyethylene glycol dimethacrylate:PEGDMA

  8. Materials Initiators Ammonium persulfate:APS Cumene hydroperoxide:CHP Tetramethyl ethylenediamine:TMEDA

  9. Fabrication Sequence of MIP Preparation of MIP crush of MIP Washing with 20 wt.% methanol and drying of MIP Screening of MIP

  10. Results and discussion

  11. HPLC analysis of protein mixture

  12. Typical calibration curve of Cytochrome C by using HPLC

  13. Typical calibration curve of Albumin by using HPLC

  14. Typical calibration curve of Lysozyme by using HPLC

  15. Typical calibration curve of Myoglobin by using HPLC

  16. Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-TEGDMA Albumin 4.5% Water 10.4% DMAPMA 5.0% TEGDMA 80% APS,TMEDA Selectivity: 1:1.9:14.2:1.4

  17. Selectivity mass ratio to adsorbent of M.I.P Sample name: MAA-TEGDMA Albumin 3.1% Water 7.3% MAA 2.7% TEGDMA 86.8% APS,TMEDA Selectivity: 52.2:160.1:1:77.5

  18. Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 1 Albumin 2.4% Water 9.2% AM 2.4% TEGDMA 86% APS,TMEDA Selectivity: 1:4.1:6.5:2.5

  19. Selectivity mass ratio to adsorbent of M.I.P Sample name: AM-TEGDMA 2 Albumin 2.4% Water 11.2% AM 2.3% TEGDMA 84% CHP,TMEDA Selectivity 4.4:25.8:8.9:1

  20. Selectivity mass ratio to adsorbent of M.I.P Sample name: HEMA-PEGDMA Albumin 4.5% Water 10.5% HEMA 5.1% PEGDMA 80% APS,TMEDA Selectivity 1: 4.3: 1: 1.8

  21. Selectivity mass ratio to adsorbent of M.I.P Sample name: DMAPMA-PEGDMA Albumin 5.4% Water 12.6% DMAPMA 6.0% PEGDMA 76% APS,TMEDA Selectivity 3.1:6.4:5.4:1

  22. Mass ratio of Adsorbent for different composition MIP

  23. Conclusions The DMAPMA-TEGDMA MIP can obtain high selectivity for albumin 2.The mass ratio of DMAPMA-TEGDMA MIP for adsorbent = Albumin : Lysozyme : Myoglobin : Cytochrome C = 14.2 : 1.9 : 1.4 : 1

  24. Thank you for your attention

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