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Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules

Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules. Danny Muslin. Need. Approximately 90% of nitrogen that is fixed through natural processes is carried out by free-living bacteria such as Rhizobium (AG West Biotech). Model Organisms. Organisms used will be:

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Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules

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  1. Using Iron-rich Fertilizer to increase Hemoglobin in Rhizobium Nodules Danny Muslin

  2. Need • Approximately 90% of nitrogen that is fixed through natural processes is carried out by free-living bacteria such as Rhizobium (AG West Biotech)

  3. Model Organisms • Organisms used will be: • Soy Bean Plant • Rhizobium leguminosarum

  4. Nitrogen Cycle

  5. Rhizobium • Rod shaped Gram -ve bacteria • Fixes nitrogen after infecting host plants • Has a mutually beneficial relationship with the host

  6. Rhizobium Nodules • Inside the nodules is where nitrogen fixation occurs • Nodules have a finite lifespan. (in the case of soybean, estimated at 50-60 days )

  7. Hemoglobin • An iron-containing oxygen-transport metalloprotein Depiction of hemoglobin in a human adult

  8. Iron Increases hemoglobin Production • (2008, Ginzburg et al.,) Exogenous iron increases hemoglobin production in beta-thalassemic mice • (1960, Hammond) Extra iron supplements can be utilized by infants to increase hemoglobin concentration

  9. Chelated Iron • Iron that has undergone chelation • Easier for plants to absorb

  10. Method of Testing Hemoglobin in Rhizobium Nodules • Method used will be: Cyanmethemoglobin Method for Determining Hemoglobin Concentration 

  11. Do-ability • Can easily obtain soy beans with the leguminosarum rhizobium • Can easily buy iron chelated fertilizer • Have a spectrophotometer in the lab

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