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Aim: How do scientists identify people using DNA Fingerprinting?

Aim: How do scientists identify people using DNA Fingerprinting?. LO: SWBAT explain how scientists identify people using DNA fingerprinting DN: Why do we fingerprint people? What is it useful for? HW: read pp 322-325, page 339 #18-19 Fri: Review Book- Respiration

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Aim: How do scientists identify people using DNA Fingerprinting?

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  1. Aim: How do scientists identify people using DNA Fingerprinting?

  2. LO: SWBAT explain how scientists identify people using DNA fingerprinting DN: Why do we fingerprint people? What is it useful for? HW: read pp 322-325, page 339 #18-19 Fri: Review Book- Respiration Quarterly- Next Wed/Thurs

  3. Why do we Fingerprint? So we can identify people

  4. Why is DNA used to identify people? People have different DNA base sequences, therefore each individual will have a different DNA fingerprint.

  5. Genetic Markers: ATTGCCTAGG - Individual 1 ATTGCCATGG - Individual 2 • particular stretches of ____ that are _________ among individuals. DNA variable • Our __________ regions are highly _________ from one individual to another and serve as good ________________ to be used for DNA fingerprinting. noncoding variable genetic markers

  6. How do scientists make a DNA Fingerprint? Investigators need to find some ____ of the perpetrator. DNA Where can the investigators find the DNA? • Blood • Hair • Nails • Skin

  7. Only a small amount of DNA is needed! Polymerase Chain Reaction: PCR The sample of DNA is amplified (___________) multiplied

  8. PCR Animation

  9. Gel Electrophoresis • Amplified DNA is cut with specific __________________. • 2) DNA segments are loaded into wells in a porous ____. • 3) Electric current is passed through the gel making one side of the gel __ charged and one side __ charged. restriction enzymes gel – +

  10. Gel Electrophoresis • DNA is ___________ charged & moves through the gel to the _________ side. • 5) Smaller segments move _______ & ________ than the larger segments. negatively positive faster farther

  11. Gel Electrophoresis 6) The gel is then ________ so we can see the DNA ________ patterns. stained banding Result: The smaller segments of DNA are found closer to the _________ end than the larger segments. positive

  12. **Separates DNA segments according to _____. SIZE

  13. Gel Electrophoresis - +

  14. DNA Fingerprint Questions: • What is the charge of DNA? • Where would we find the smallest DNA fragments, closer to the + end or the – end? • Why do we get different banding patterns for each individual? • 4) Which suspect was at the crime scene?

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