1 / 5

Procedures for the unknown

Procedures for the unknown. Streak your unknown mixture (after shaking) on 2 TP plates (one at 30 and 1 at 37 C) 2)Start constructing your Dichotomous key:

colman
Download Presentation

Procedures for the unknown

An Image/Link below is provided (as is) to download presentation Download Policy: Content on the Website is provided to you AS IS for your information and personal use and may not be sold / licensed / shared on other websites without getting consent from its author. Content is provided to you AS IS for your information and personal use only. Download presentation by click this link. While downloading, if for some reason you are not able to download a presentation, the publisher may have deleted the file from their server. During download, if you can't get a presentation, the file might be deleted by the publisher.

E N D

Presentation Transcript

  1. Procedures for the unknown • Streak your unknown mixture (after shaking) on 2 TP plates (one at 30 and 1 at 37 C) 2)Start constructing your Dichotomous key: A dichotomous key is a way of dividing groups of organism. Bacteria are categorized using this method based on differences in their physical or metabolic attributes. Example: http://sites.google.com/site/scienceprofonline/virtualmicrobiologyclasslaboratory233

  2. Isolation of different colony types

  3. Procedures for the unknown 3) Isolate the 2 different morphological types of colonies each in a separate streak on 1 NA plate (you may use the stereo microscope), until both cultures are completely PURE. Give each colony a name. 4) Streak both colonies on Mac Conkey and EMB plates 5) Take records of you results from each media, including TP (Make a table) (Colony types, color, pigment production, etc)

  4. Procedures for the unknown 6) Gram stain. Record: • Gram reaction • Cell morphology (Rods or cocci) 6) Follow Dichotomous key to perform morphological and physiological tests to get to the identification of both strains

  5. Procedures for the unknown Tools: • List of putative unknowns • Chart with biochemical characteristics of bacteria • Examples of DK

More Related