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Learn about cellular division in both prokaryotes and eukaryotes, including the process of DNA replication, chromosome structure, and the stages of mitosis. Understand the importance of cell division for growth, repair, and reproduction.
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Cell Division • All cells are derived frompre-existingcells • New cells are produced for growth and to replacedamaged or old cells • Differs inprokaryotes(bacteria) andeukaryotes(protists, fungi, plants, & animals)
Keeping Cells Identical Theinstructions for making cell partsare encoded in the DNA, so each new cell must get acomplete set of the DNA molecules
DNA Replication • DNA must be copied orreplicated before cell division • Each new cell will then have anidentical copyof the DNA Original DNA strand Two new, identical DNA strands
Identical Daughter Cells Two identical daughter cells Parent Cell
Prokaryotic Chromosome • The DNA of prokaryotes (bacteria) is one, circular chromosomeattached to the inside of the cell membrane
Eukaryotic Chromosomes • All eukaryotic cells store genetic information in chromosomes • Most eukaryotes have between 10 and 50 chromosomes in their body cells • Human body cells have 46 chromosomes or 23 identical pairs
Eukaryotic Chromosomes • Each chromosome is composed of a single,tightly coiled DNA molecule • Chromosomes can’t be seen when cells aren’t dividingand are called chromatin
Compacting DNA into Chromosomes • DNA is tightly coiled around proteins called histones
Chromosomes in Dividing Cells • Duplicated chromosomes are called chromatids & are held together by the centromere Called Sister Chromatids
Types of Cell Reproduction • Asexual reproduction involves a single cell dividing to make 2 new, identical daughter cells • Mitosis & binary fission are examples of asexual reproduction • Sexual reproduction involves two cells (egg & sperm) joining to make a new cell (zygote) that is NOT identical to the original cells • Meiosis is an example
Cell Division in Prokaryotes • Prokaryotes such as bacteria divide into 2 identical cells by the process of binary fission • Single chromosome makes a copy of itself • Cell wall forms between the chromosomes dividing the cell Parent cell Chromosome doubles Cell splits 2 identical daughter cells
Five Phases of the Cell Cycle • G1 - primary growth phase • S – synthesis; DNA replicated • G2 - secondary growth phase collectively these 3 stages are called interphase • M - mitosis • C - cytokinesis
Interphase - G1 Stage • 1st growth stage after cell division • Cells mature by making more cytoplasm & organelles • Cell carries on its normal metabolic activities
Interphase – S Stage • Synthesis stage • DNA is copied or replicated Two identical copies of DNA Original DNA
Interphase – G2 Stage • 2nd Growth Stage • Occurs after DNA has been copied • All cell structures needed for division are made (e.g. centrioles) • Both organelles & proteins are synthesized
What’s Happening in Interphase? What the cell looks like Animal Cell What’s occurring
Sketch the Cell Cycle DNA Copied Cells prepare for Division Cells Mature Daughter Cells Cell Divides into Identical cells
Mitosis • Division of the nucleus • Also called karyokinesis • Only occurs in eukaryotes • Has four stages • Doesn’t occur in some cells such as brain cells
Four Mitotic Stages • Prophase • Metaphase • Anaphase • Telophase
Early Prophase • Chromatin in nucleus condenses to form visible chromosomes • Mitotic spindle forms from fibers in cytoskeleton or centrioles (animal) Cytoplasm Nucleolus Nuclear Membrane Chromosomes
Late Prophase • Nuclear membrane & nucleolus are broken down • Chromosomes continue condensing & are clearly visible • Spindle fibers called kinetochoresattach to the centromere of each chromosome • Spindle finishes forming between the poles of the cell
Late Prophase Chromosomes Nucleus & Nucleolus have disintegrated
Spindle Fiber attached to Chromosome Kinetochore Fiber Chromosome
Spindle Fibers • The mitotic spindle form from the microtubulesin plants and centrioles in animal cells • Polar fibers extend from one pole of the cell to the opposite pole • Kinetochore fibers extend from the pole to the centromere of the chromosome to which they attach • Asters are short fibers radiating from centrioles
Metaphase • Chromosomes, attached to the kinetochore fibers, move to the center of the cell • Chromosomes are now lined up at the equator Equator of Cell Pole of the Cell
Metaphase Asters at the poles Spindle Fibers Chromosomes lined at the Equator
Metaphase Aster Chromosomes at Equator
Anaphase • Occurs rapidly • Sister chromatids are pulled apart to opposite poles of the cell by kinetochore fibers
Anaphase Sister Chromatids being separated
Telophase • Sister chromatids at opposite poles • Spindle disassembles • Nuclear envelope forms around each set of sister chromatids • Nucleolus reappears • CYTOKINESIS occurs • Chromosomes reappear as chromatin
Cytokinesis • Means division of the cytoplasm • Division of cell into two, identical halves called daughter cells • In plant cells, cell plate forms at the equator to divide cell • In animal cells, cleavage furrow forms to split cell
Cytokinesis Cleavage furrow in animal cell Cell plate in animal cell
Daughter Cells of Mitosis • Have the same number of chromosomes as each otherand as the parent cell from which they were formed • Identical to each other, but smaller than parent cell • Must grow in size to become mature cells (G1 of Interphase)
Identical Daughter Cells What is the 2n or diploid number? 2 Chromosome number the same, but cells smaller than parent cell
Eukaryotic Cell Division • Used for growth and repair • Produce two new cells identical to the original cell • Cells are diploid (2n) Chromosomes during Metaphase of mitosis Cytokinesis Anaphase Prophase Metaphase Telophase
Mitosis in Onion Root Tips Do you see any stages of mitosis?
Facts About Meiosis • Preceded by interphase which includeschromosome replication • Twomeiotic divisions --- Meiosis I and Meiosis II • Called Reduction- division • Original cell is diploid (2n) • Four daughter cellsproduced that are monoploid (1n)
Facts About Meiosis • Daughter cellscontain half the number of chromosomesas the original cell • Producesgametes(eggs & sperm) • Occurs in the testes in males (Spermatogenesis) • Occurs in the ovaries in females (Oogenesis)
More Meiosis Facts • Start with 46 double stranded chromosomes (2n) • After 1 division - 23 double stranded chromosomes (n) • After 2nd division - 23 single stranded chromosomes (n) • Occurs in our germ cells that produce gametes