Next Generation Sequencing

1. The Application of Next Generation Sequencing Compared with the traditional sequencing technology, the next generation sequencing (NGS) technology has strong advantages of ultra-high speed, high throughput, low cost and high efficiency. The development of this emerging technology and the establishment of a new generation of sequencing platform have had a significant impact on genome research, human health and social cognition. The book consists of five parts and 18 chapters.The first part and the second part respectively summarize the working principle, method and characteristics of the traditional sangerDNA sequencing and the new generation sequencing platform.The third part analyzes the bottleneck of sequencing technology and its solution.The fourth part introduces the commercial application of sequencing and the emerging sequencing platform.The fifth part discusses the wide application of the new generation sequencing technology in genomics research."Next-generation genome sequencing: toward personalized medicine," written by researchers and inventors involved in the development and application of NGS technology, is the world's first book to introduce the new generation of DNA sequencing technology.It can be used as a reference book for teachers, students and researchers in the fields of biology, medicine and agronomy in colleges and universities. It can also enlighten and help readers who want to understand personal genome information, personalized medicine, ethics and other issues. DNA sequencing technology research, development and progress, in all the research field of life science shows its special charm. In this paper, combing the development of DNA sequencing technology pulse road, the first generation of sequencing technology was introduced in detail the sanger a library, chemical degradation and Roche 454 sequencing method of the second

2. generation sequencing technology, Illumina Solexa sequencing method, the ABISOLID sequencing method, and the third generation sequencing technology of single molecule fluorescence sequencing method, the nanopore sequencing method, and analyzes Next Generation Sequencing and the characteristics of sequencing technology, points out that the generation of DNA sequencing technology and the rapid development of life science research has brought the revolutionary change, make the human to nature and their own cognitive entered to a new scientific level. New generation sequencing technologies (NGS) can generate high-throughput rna-seq data and further study the transcriptome, including 454, illumina, SOLiD and Ion Torrent technologies, and advanced single-molecule long-reads sequencing methods, including PacBio and nano-pore sequencing (nanopore sequencing). The ease of use of high-throughput sequencing has long noncoding (IncRNA) to identify possible, according to locate these IncRNA genome can be divided into gene, gene, between sense and antisense and two-way IncRNA, IncRNA transcription in the plant through the polymerase II, IV and V, orientation of DNA methylation and RNA (RdDM) targeting epigenetic regulation(regulate gene expression through histone modification, chromosome annularization and other mechanisms). The advantages of NGS is fast, repeatable and high-precision sequencing technology.