leucine rich alpha 2 glycoprotein

1. SPECIFICATION SHEET Rev 111214PAB-YSS022 Rabbit Anti-Rat LRGPolyclonal Antibody Rabbit, Polyclonal (LRG) Cat. No. DPAB2488RR Lot. No. (See product label) PRODUCT INFORMATION ANTIGEN BACKGROUND Product Overview: Rabbit anti-rat leucine-rich alpha-2- glycoprotein polyclonal antibody. Antigen Description: Among the diseases which should be distinguished from iNPH (idiopathic normal pressure hydro- cephalus) of elderly people showing symptoms such as gait disorder and dementia, there are some neurodegenerative diseases such as Alzheimer's disease, FTLD (frontotemporal lobar degeneration) and disorder associated with Parkinson's disease. iNPH can be treated by ventriculo- peritoneal shunt (VP shunt), so a marker in spinal fluid which can distinguish it from neurodegenerative diseases in an early stage has been required. Arai, Miyajima et al. had identified the high level of leucine-rich alpha-2-glycoprotein (LRG) in cerebrospinal fluid of iNPH patients, and they re- ported that measurement of LRG would be useful for differ- ential identification of iNPH. This antibody shows positive signals with choroids plexus cells and cilia of ependymal cells of rat. Immunogen: Synthetic peptide of a part of Rat LRG (LVLRENQLQEASARWLQG) Host animal: Rabbit Species: Rat Purification:Purified with antigen peptide. Form: Lyophilized product from 1% BSA in PBS containing 0.05%NaN3 Applications: This antibody can be used for immunohisto- chemistry with formalin fixed paraffin embedded tissues by several techniques such as Avidin Biotin Complex (ABC) Method or used for immunofluorescent staining (e.g. Al- exa488) with frozen secsion 30 μm thick . The optimal con- centration is about 1-5 μg/mL however, the concentration should be optimized by each laboratory.This antibody can be used for western blotting in concentration of 1 - 5 μg /mL. Specificity: Rat LRG specific. Dilute: 1.0 mL deionized water will be added to the product (the conc. comes up 100 μg /mL) Storage: Lyophilized product, 5 years at 2 - 8 °C; Solution, 2 years at -20 °C. Introduction: The protein, LRG, or leucine-rich alpha-2- glycoprotein, appears to be a specific marker of local inflam- mation. The researchers saw that LRG had the best perform- ance in terms of specificity (how much it can distinguish those patients with appendicitis from those without it) and sensitivity (how well it serves as a marker to detect the disease). Levels of the LRG protein are markedly elevated in acute appendici- tis and therefore could be used as a diagnostic aid. Keywords: LRG; leucine-rich alpha-2-glycoprotein; LRR; leucine-rich repeat REFERENCES 1. Lins H, Wichart I, Bancher C, Wallesch CW, Jellinger KA, Rösler N. Immunoreactivities of amyloid beta peptide((1- 42)) and total tau protein in lumbar cerebrospinal fluid of patients with normal pressure hydrocephalus. J Neural Transm. 2004 Mar; 111 (3): 273-280. 2. Li X, Miyajima M, Mineki R, Taka H, Murayama K, Arai H. Analysis of potential diagnostic biomarkers in cerebrospi- nal fluid of idiopathic normal pressure hydrocephalus by proteomics. Acta Neurochir (Wien). 2006 Aug; 148 (8): 859-864. 3. O'Donnell LC, Druhan LJ, Avalos BR (September 2002). "Molecular characterization and expression analysis of leucine-rich alpha2-glycoprotein, a novel marker of granu- locytic differentiation". J. Leukoc. Biol. 72 (3): 478-485. Creative Diagnostics. All rights reserved. 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com