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mouse fibronectin elisa kit

Human Fibronectin ELISA Kit can be provided from Creative Diagnostics.<br>https://www.creative-diagnostics.com/Human-Fibronectin-EIA-Kit-123354-471.htm<br>

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mouse fibronectin elisa kit

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  1. Human Fibronectin ELISA Kit Cat. No.:DEIA6909 Pkg.Size:96T Intended use The Human Fibronectin ELISA Kit measures human fibronectin from both plasma and cellular extracts. General Description Fibronectin, a major cell surface glycoprotein, is found on cell surfaces and circulating in plasma. It has been shown to be important in wound healing and in cellular interactions. Reagents And Materials Provided The kit is in a 96-well (precoated strip) format and includes all the necessary reagents and a complete protocol to perform the assay. Storage Do Not Freeze Assay Steps 1. Add 100ul samples or standard in duplicate, add 50ul of Fibronectin Ab. Incubate at 37ºC. 2. Add 50ul alkaline phosphatase HFN conjugate. Incubate at 37ºC for 1 hour. 3. Aspirate and wash (3×). 4. Add 200ul Substrate, incubate at 37ºC. Read Absorbance at 410nm. Typical Standard Curve See Figure 1. Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com 1

  2. Evaluation A number of other species of fibronectin have been evaluated with the kit. Detection Range 25-2000 ng/ml Sensitivity The range of these other species' assays is less sensitive. Specificity Bovine Fibronectin is 0.5-100ug/ml, Mouse Fibronectin is 0.2-50ug/ml, and Rat Fibronectin is 0.2-20ug/ml. Analyte Gene Information Gene Name FN1 fibronectin 1 [ Homo sapiens ] FN1 FN1; fibronectin 1; fibronectin; CIG; cold insoluble globulin; FINC; GFND2; LETS; migration stimulating factor; MSF; cold-insoluble globulin; migration-stimulating factor; FN; FNZ; ED-B; GFND; DKFZp686H0342; DKFZp686I1370; DKFZp686F10164; DKFZp686O13149; Official Symbol Synonyms GeneID 2335 Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com 2

  3. mRNA Refseq NM_002026 Protein Refseq NP_002017 MIM 135600 P02751 UniProt ID Chromosome Location 2q34 Amoebiasis, organism-specific biosystem; Amoebiasis, conserved biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Bacterial invasion of epithelial cells, organism- specific biosystem; Bacterial invasion of epithelial cells, conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; ECM-receptor interaction, organism-specific biosystem; collagen binding; extracellular matrix structural constituent; heparin binding; protein binding; Pathway Function REFERENCES 1. Hess L.W., O’Brien W.F., Holmberg J.A., Winkel C.A., Monaghan W.P., and Hemming V.G. Plasma and Amniotic Fluid Concentrations of Fibronectin During Normal Pregnancy. Obstetrics & Gynecology, 68: 25-28 (1986). 2. Eielson C., Kaplan D., Mitnick M.A., Paliwal I., and Insogna K. Estrogen Modulates PTH-Induced Fibronectin Production in Human and Rat Osteoblast-like Cells. Endocrinology 135, No. 4: 1639-1644 (1994). Creative Diagnostics. All rights reserved 45-16 Ramsey Road Shirley, NY 11967, USA Tel: 631-624-4882 ·Fax:631-614-7828 E-mail: info@creative-diagnostics.com www.creative-diagnostics.com 3

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