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Total oocyte fluorescence

hMad2-GFP (oocyte 1 in C, D). hMad2-GFP + Mad2MO (oocytes 2 & 3 in C, D). C. 1. 2. 3. D. 04:00. 08:00. 16:00. GV. 12:00. 5 ' —. C. A. T. G. G. C. A. C. A. G. C. A. G. C. T. C. G. C. C. G. A. G. A. G. C. — 3 '. Mad2a. 5 ' —. A. T. G. G. C. G. C. T.

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Total oocyte fluorescence

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  1. hMad2-GFP (oocyte 1 in C, D) hMad2-GFP + Mad2MO (oocytes 2 & 3 in C, D) C 1 2 3 D 04:00 08:00 16:00 GV 12:00 5'— C A T G G C A C A G C A G C T C G C C G A G A G C — 3' Mad2a 5'— A T G G C G C T G C A G C T C T C C C G G G A G C — 3' hMad2 A B Total oocyte fluorescence Time (h post-GVBD) E hMad2-GFP mRNA (15) hMad2-GFP mRNA + Mad2MO (47) Concentration of hMad2-GFP (nM, mean  S.E.M.) (21) (35) GV 16 h post- GVBD GV 16 h post- GVBD Supplemental Fig_4 Homer et al

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