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Bacteria Associated with Periodontal Disease

Bacteria Associated with Periodontal Disease. 23 June 2008 Article Presentation BBSI Summer 2008. Annica Stull-Lane. VCU Mentor: Kimberly Jefferson. Diaz PI, Zilm PS, Rogers AH. (2002) Microbiol 148 : 467-472. How do bacteria cause disease?. ?. !. DISEASE.

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Bacteria Associated with Periodontal Disease

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  1. Bacteria Associated with Periodontal Disease 23 June 2008 Article Presentation BBSI Summer 2008 Annica Stull-Lane VCU Mentor: Kimberly Jefferson Diaz PI, Zilm PS, Rogers AH. (2002) Microbiol 148: 467-472

  2. How do bacteria cause disease? ? ! DISEASE http://www.ou.edu/class/pheidole/bacteria.html

  3. What causes periodontal disease? • Periodontal disease: healthy gums gingivitis periodontitis http://www.enexus.com/gumdisease/ • Well, associated with many bacteria, including…

  4. BACTERIA! • Strictly anaerobic • Found in oral cavity Porphyromonas gingivalis Fusobacterium nucleatum WAIT! http://www.pgingivalis.org/photo_gallery.htm http://www.ronaldschulte.nl/Preparaten%20Tandplaque%2002.htm

  5. Experimental Question • Diaz PI, Zilm PS, Rogers AH. (2002) Microbiol 148: 467-472 • Background: Bradshaw et al. (1998) suggested F. nucleatum as a “bridge” or “mediator” of co-aggregation • Diaz et al. ask: Can F. nucleatum by itself protect P. gingivalis (and perhaps other anaerobes less tolerant to oxygen)?

  6. Overview of Experiments • Test oxygen tolerance of monocultures and a co-culture • Test carbon dioxide requirement of monocultures and co-culture P. gingivalis Co-culture Monocultures Chemostat F. nucleatum

  7. Co-culture Experiment • cell viability measured by viable counts 0 % O2 10 % O2 20 % O2 5 % CO2 0 % CO2

  8. Results: Monocultures • Oxygen tolerance • P. gingivalis: doesn’t survive at 10% O2 or 20% O2 , but does at 3% and 6% O2 • F. nucleatum survives at even higher O2 concentrations than air (21-ish% O2)

  9. Results: Monocultures • CO2 requirement P. gingivalis F. nucleatum

  10. Results: Co-culture • In the presence of F. nucleatum, With CO2 P. gingivalis survived in all oxygen concentrations! Without CO2 P. gingivalis survived w/o CO2 in all O2concentrations!

  11. Results: Co-culture • At the higher O2 level (20%): 0% O2 20% O2 A biofilm formed, w/ P. gingivalis F. nucleatum cells lengthened

  12. So how do these anaerobic bacteria survive? • This study suggests: • F. nucleatum creates a necessary reduced O2 condition and a supply of CO2 for survival of P. gingivalis • O2 tolerances, oxidative stress and biofilm, CO2 requirements and co-culture • Plaque biofilm gives insight into relationships among pathogenic bacteria

  13. Etiology of periodontal diseases • Important: determining how microorganisms survive during different stages of plaque development • Control of particular species might drastically affect the pathogenic ecosystem periodontal DISEASE

  14. Diaz PI, Zilm PS, Rogers AH. Fusobacterium nucleatum supports the growth of Porphyromonas gingivalis in oxygenated and carbon-dioxide-depleted environments (2002) Microbiol 148: 467-472

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