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Immunological algorythms in solid organ transplantation and bone marrow transplantation

Fundeni Centre for Immunogenetics & Virology. Immunological algorythms in solid organ transplantation and bone marrow transplantation. Ileana Constantinescu. Fundeni Centre for Immunogenetics & Virology. VIROLOGICAL ASSESSMENT

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Immunological algorythms in solid organ transplantation and bone marrow transplantation

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  1. Fundeni Centre for Immunogenetics & Virology Immunological algorythms in solid organ transplantation and bone marrow transplantation • Ileana Constantinescu

  2. Fundeni Centre for Immunogenetics & Virology VIROLOGICAL ASSESSMENT Both donor and recipient are tested for: VHB, VHD, VHC, HIV 1/2, CMV, EBV, HSV 1 and 2, VZV, HTLV 1/2 , rubella virus, toxoplasma gondii and chlamydia. Methods Indirect diagnostic tests(serological) Direct diagnostic tests, molecular biology tests (PCR, RT-PCR).

  3. Fundeni Centre for Immunogenetics & Virology IMMUNOGENETICS HLA Typing by molecular biology methods – PCR SSOP- sequence-specific oligonucleotide probe hybridization (medium resolution ) SSP – sequence-specific primers (high resolution) SBT – (the highest available resolution) KIR Genotyping SSP  Cytokine gene polymorphisms SSP Cross- match - CDC AHG - ELISA - LUMINEXAnti-HLA antibody detection and identification - CDC - ELISA  - LUMINEX

  4. Fundeni Centre for Immunogenetics & Virology Bone Marrow Transplantation • HLA cls I and II SSP high resolution • HLA SBT – A, B, C, DRB1, DQB1, DPB1 • Crossmatch CDC, ELISA, Luminex • KIR genotyping • Cytokine gene polymorphisms

  5. Fundeni Centre for Immunogenetics & Virology Liver transplantation • HLA cls I and II – SSO low medium resolution • Crossmatch – CDC, ELISA, Luminex • Cytotoxic antibody- screening - identification

  6. Fundeni Centre for Immunogenetics & Virology Heart transplantation • HLA cls I and II – SSP medium resolution - SSO low medium resolution • Crossmatch – CDC, ELISA, Luminex • Cytotoxic antibody (ELISA, Luminex) - screening - identification

  7. Fundeni Centre for Immunogenetics & Virology Cornea transplantation • HLA cls I and II – SSP medium resolution • HLA cls I and II – SSO low medium resolution • Crossmatch – ELISA, Luminex

  8. Fundeni Centre for Immunogenetics & Virology EQAs • UK NEQAS • Balkan EPT scheme • Medical Univ. of Wienna EPT scheme • National EPT scheme for HLA DNA

  9. Fundeni Centre for Immunogenetics & Virology Conf. Dr. Ileana Constantinescu Seful Centrului de Imunogenetica si Virusologie Institutul Clinic Fundeni Sos. Fundeni, Nr.258, sector 2, Bucuresti Tel/Fax: 021 318 04 48 Interior: 021 318 04 00 /int. 2276 Mobil: 0744 341 984 e-mail: ileanasci@yahoo.com constantinescu.ileana@gmail.com Web-site:www.imunogenetica.ro

  10. MULTUMESC !

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