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Module 1B

2. General Biosafety Module 1 Outline. OverviewModule 1ABiosafety Level 1 and 2 LabsModule 1BSafety EquipmentModule 1CDecontamination and Biohazardous Waste HandlingModule 1D . 3. General Biosafety Module 1B Synopsis. Information about this training moduleFor a comment or to ask a question,

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Module 1B

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    1. Module 1B

    2. 2 General Biosafety Module 1 Outline Overview Module 1A Biosafety Level 1 and 2 Labs Module 1B Safety Equipment Module 1C Decontamination and Biohazardous Waste Handling Module 1D

    3. 3 General Biosafety Module 1B Synopsis Information about this training module For a comment or to ask a question, you will find a link on the main page of the course. To enhance the size of this PowerPoint presentation, click on the “screen icon” at the bottom right of this page Once this section is completed, click on the “X” and the top right of the window to close it and return to the main page to access Module 1C.

    4. 4 Who needs this training? Individuals who work with or have access to biohazardous materials.

    5. 5 Biosafety Levels of Containment Containment involves a combination of laboratory issues to include: Standard microbiological practices Personal protective equipment Safety equipment Primary barrier Laboratory facilities Secondary barrier

    6. 6

    7. 7 BSL-1 - Overview Suitable for work involving well- characterized agents not known to cause disease in healthy adult humans and of minimal potential hazard to laboratory personnel and the environment.

    8. 8 Examples of Organisms Requiring BSL-1 Bacillus subtilis Saccharomyces cerevisiae Staphylococcus epidermidis Escherichia coli K-12 (NIH Guidelines, Appendix B) (all are risk group 1 organisms)

    9. 9 BSL-1 Standard Microbiological Practices Restrict or limit access when conducting research Prohibit eating, drinking and smoking Prohibit mouth pipetting

    10. 10 BSL-1 Standard Microbiological Practices Needles & sharps precautions Use sharps containers DON’T break, bend, re-sheath or reuse syringes or needles Use alternatives to needles when available

    11. 11 BSL-1 Standard Microbiological Practices Needles & sharps precautions (cont.) DON’T place needles or sharps in office waste containers

    12. 12 BSL-1 Standard Microbiological Practices Use mechanical pipetting devices

    13. 13 BSL-1 Standard Microbiological Practices Wash hands

    14. 14 BSL-1 Standard Microbiological Practices Minimize splashes and aerosols Decontaminate work surfaces daily Decontaminate infectious waste Maintain an insect & rodent control program

    15. 15 BSL-1 Safety Equipment (Primary Barrier) Personal protective equipment Face shield Eye wear Gloves Lab coat

    16. 16 BSL-1 Facility Design (Secondary Barrier) Countertops and floors easily cleaned and decontaminated

    17. 17 Requirements: Lab Location -not separated from other lab sections Structure -normal construction Ventilation -no specific requirements BSL-1 Facility Design (Secondary Barrier)

    18. 18 Supervisor Trained in microbiological or related science practices Lab personnel Specific training in lab procedures BSL-1 Training Requirements

    19. 19

    20. 20 BSL-2 - Overview Suitable for work involving agents of moderate potential hazard to personnel and the environment.

    21. 21 Examples of Organisms Requiring BSL-2 Measles virus Salmonella spp. Toxoplasma spp. Hepatitis B virus (NIH Guidelines, Appendix B-II) (all are risk group 2 organisms.)

    22. 22 BSL-2 Standard Microbiological Practices Follow all practices used for BSL-1 containment

    23. 23 BSL-2 Safety Equipment (Primary Barrier) Class II Biological Safety Cabinet To protect product, personnel, and the environment. But….only recommended Not required for BSL-2 work

    24. 24 BSL-2 Safety Equipment (Primary Barrier) Class II Biological Safety Cabinet Equipment is laid out to not restrict airflow in the cabinet

    25. 25 BSL-2 Facility Design (Secondary Barrier)

    26. 26 BSL-2 Facility Design (Secondary Barrier)

    27. 27 BSL-2 Facility Design (Secondary Barrier) Signage on the door with restricted access when work in progress.

    28. 28 BSL-2 Facility Design (Secondary Barrier) BSL-1 Facilities PLUS: Decon method Autoclave may be available Off-site program Eyewash station present

    29. 29 Requirements: Location - separated from public areas Structure - normal construction Ventilation - directional BSL-2 Facility Design (Secondary Barrier)

    30. 30 BSL-2 Training Requirements

    31. 31 BSL-3 and BSL-4 Containment BSL-3 containment is discussed in Module 2. BSL-4 containment is not available on the UNMC/UNO campuses.

    32. 32 Biological Spill Clean-Up All laboratories should have posted an emergency plan To handle accidental spills To handle personnel contamination UNMC policy #IBC04 is available on the IBC website for information www.unmc.edu/ibc/

    33. 33 Reporting of Research Related Adverse Events Research related adverse events include Biological spills Exposure to biohazardous agents Non-adherence to NIH Guidelines All such events must be reported to the IBC Refer to UNMC policy #IBC24 for information and a reporting form www.unmc.edu/ibc/ Biosafety Officer can be contacted for additional information

    34. 34 This completes Module 1B Click on the “X” at the top of the page to close this window. Then: Continue on with Module 1C or Comment or ask questions about Module 1B by clicking on the link on the main page of the course

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