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Fundamental Autoclave Techniques

The following information is directed at aseptic/sterile surgical, tissue culture (PC1

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Fundamental Autoclave Techniques

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    1. Fundamental Autoclave Techniques June, 2008 Veronika Tatarinoff GSBME University of NSW Brandy Nelson University of Kentucky

    2. The following information is directed at aseptic/sterile surgical, tissue culture (PC1 & PC2) and QC1 techniques at the GSBME.

    3. GSBME Autoclave Training Program This presentation forms part of the GSBME Autoclave training program. 1. Fundamental Autoclave Techniques (FAT) 2. Review of supporting literature (SOP, SWP, RA) 3. FAT quiz 4. Demonstration of GSBME Autoclave use 5. Competency assessment 6. Authorisation sign off in WITA

    4. Learning outcomes : At the end of this session participants will be able to: Describe the mechanisms of autoclave sterilisation Identify autoclave/s at GSBME Identify materials that can be autoclaved Package and label items for sterilization Correctly load/unload the autoclave Identify and select appropriate sterilisation cycle/s Verify autoclave sterilisation process Maintain log of sterilisation Dispose of biowaste appropriately Identify and use the Personal Protective Equipment (PPE) and devices in a safe manner. Identify hazards and risks to self and others, improper autoclave techniques and identify appropriate responses to injury.

    5. What does ‘aseptic’ mean? “preventing putrefaction [rotting, decomposition] by securing the absence of bacteria” Oxford Dictionary Disinfection. The process of destroying or inhibiting the growth of micro-organisms on a surface. Sterilisation. The destruction of all living micro-organisms, including viruses and spores. This is an absolute state.

    6. Micro-organisms Include bacteria, viruses, fungi & spores Found on every external surface including respiratory tract, alimentary canal A micro-organism “infection” occurs when the micro-organism grows in an area where it isn’t normally found or in numbers greater than normal. Surgical and cell culture procedures provide an ideal opportunity for micro-organisms to cause problems.

    7. Sterilisation methods There are a number or methods which can kill or remove micro-organisms including: Heat (dry or steam – autoclave) Gas (eg Ethylene Oxide) UV or gamma irradiation H202 plasma Liquid sterilisation (eg glutaraldehyde) Filter sterilisation (fluids only)

    8. Fundamental autoclave information Personal Protective Equipment (PPE) Packaging Loading / Unloading Operating cycles User logs Hazards Improper Autoclave Practices

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