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The following information is directed at aseptic/sterile surgical, tissue culture (PC1
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1. Fundamental Autoclave Techniques June, 2008
Veronika Tatarinoff
GSBME University of NSW
Brandy Nelson
University of Kentucky
2. The following information is directed at aseptic/sterile surgical, tissue culture (PC1 & PC2)and QC1 techniques at the GSBME.
3. GSBME Autoclave Training Program This presentation forms part of the GSBME Autoclave training program.
1. Fundamental Autoclave Techniques (FAT)
2. Review of supporting literature (SOP, SWP, RA)
3. FAT quiz
4. Demonstration of GSBME Autoclave use
5. Competency assessment
6. Authorisation sign off in WITA
4. Learning outcomes : At the end of this session participants will be able to:
Describe the mechanisms of autoclave sterilisation
Identify autoclave/s at GSBME
Identify materials that can be autoclaved
Package and label items for sterilization
Correctly load/unload the autoclave
Identify and select appropriate sterilisation cycle/s
Verify autoclave sterilisation process
Maintain log of sterilisation
Dispose of biowaste appropriately
Identify and use the Personal Protective Equipment (PPE) and devices in a safe manner.
Identify hazards and risks to self and others, improper autoclave techniques and identify appropriate responses to injury.
5. What does ‘aseptic’ mean?“preventing putrefaction [rotting, decomposition] by securing the absence of bacteria” Oxford Dictionary Disinfection.
The process of destroying or inhibiting the growth of micro-organisms on a surface.
Sterilisation.
The destruction of all living micro-organisms, including viruses and spores. This is an absolute state.
6. Micro-organisms Include bacteria, viruses, fungi & spores
Found on every external surface including respiratory tract, alimentary canal
A micro-organism “infection” occurs when the micro-organism grows in an area where it isn’t normally found or in numbers greater than normal.
Surgical and cell culture procedures provide an ideal opportunity for micro-organisms to cause problems.
7. Sterilisation methods There are a number or methods which can kill or remove micro-organisms including:
Heat (dry or steam – autoclave)
Gas (eg Ethylene Oxide)
UV or gamma irradiation
H202 plasma
Liquid sterilisation (eg glutaraldehyde)
Filter sterilisation (fluids only)
8. Fundamental autoclave information Personal Protective Equipment (PPE) Packaging Loading / Unloading Operating cycles User logs Hazards Improper Autoclave Practices