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Mycobacterium avium infection in a Horse N07-506

Mycobacterium avium infection in a Horse N07-506. Ian Hawkins. N07-506 - History. An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis Improved on banamine and lidocaine until 8/25/07. Abdominocentesis revealed elevated WBC count and TP

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Mycobacterium avium infection in a Horse N07-506

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  1. Mycobacterium avium infection in a HorseN07-506 Ian Hawkins Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  2. N07-506 - History • An 8 year old male QH gelding presented to the UFVMC on 8/18/07 with suspected anterior enteritis • Improved on banamine and lidocaine until 8/25/07. • Abdominocentesis revealed elevated WBC count and TP • Surgery performed on 8/26/07 revealed multiple jejunal and ileal masses and necrotic section of jejunum • Due to poor prognosis, the patient was euthanized • Necropsy was performed on 8/27/07 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  3. Gross Findings • Gross Diagnosis: • Granulomatous enteritis, chronic, diffuse with multifocal transmural abscess formation, small intestine. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  4. Gross Findings • Gross Diagnoses: • Renal papillary necrosis, chronic, multifocal, marked, Kidneys. • Mesenteric and colonic lymphadenomegaly. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  5. Microscopic Findings – Small intestine Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  6. Small Intestine with Acid-Fast Stain Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  7. Kidney and Liver Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  8. Mesenteric lymph node Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  9. Diagnoses • Final Anatomic Diagnoses: • Granulomatous enteritis, chronic, multifocal, severe with abundant intralesional, acid-fast bacterial rods, small intestine. • Granulomatous lymphadenitis, chronic, multifocal, moderate, mesenteric lymph node. • Granulomatous typhlocolitis, chronic, multifocal, moderate to marked, cecum and colon. • Portal hepatitis, granulomatous, chronic, multifocal, moderate to marked, liver. • Renal papillary necrosis, chronic, multifocal, marked, kidneys. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  10. Polymerase Chain Reaction • Paraffin-embedded sections of intestine were submitted to the NVSL in Ames, IA and Claus Buergelt’s lab at the University of Florida – performed by Elliot Williams. • Results from both labs were positive for Mycobacterium avium • 16sRNA positive • IS1311 positive Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  11. PCR • PCR using IS900 based primers for Mycobacterium avium ssp. paratuberculosis. • Tissue was positive using J1-J2 IS900 primers (nested primers). • Johne’s? or a variant of M. avium ssp. paratuberculosis. http://www.waterscan.co.yu/thumbnails/Mycobacterium%20avium.jpg Map N07 506 Neg Map Map nest Neg Neg N07506 P90/P91 J1/J2 Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  12. Mycobacterium avium in Horses • Infections in horses are uncommon but have been reported • Typically occur in younger adult horses and predominantly affects the gastrointestinal tract; also the lungs and liver with less frequency • Clinically horses tend to have chronic weight loss, diarrhea, and hypoproteinemia. • M. avium has been isolated from horses with Equine Granulomatous Enteritis, a sporadic idiopathic disease of young horses with similar clinical signs and lesions. Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  13. Mycobacterium infections in horses • Gross findings: • Multifocal soft to firm transmural nodules throughout affected regions of the GI tract • Ulcerated mucosa • Histopathology: • Granulomatous inflammation • High numbers of multinucleated giant cells http://freelief.com/temp/april4dee.jpg Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  14. Mycobacterium infections in horses • Johne’s Disease in a horse? • Horses have been experimentally infected with the organism; sporadic cases have been reported, but were unconfirmed and exceedingly rare. • A variant of M. avium ssp. paratuberculosis that horses maybe more susceptible to? Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  15. Acknowlegements • Barbara Sheppard, DVM, PhD, Dipl. ACVP • Elliot Williams • Giles R.G. Monif • John Roberts, DVM, Dipl. ACVP • David Taylor, BVSc, Dipl. ACVP • Fellow UF Pathology Residents • The UFVMC Histology lab staff Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

  16. Questions? Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website only

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